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EPS8L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EPS8L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112255-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EPS8L1 (NM_017729), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EPS8-like 1 | EPS8-related protein 1 | EPS8L1 | EPS8R1 | EPS8-like protein 1 | DRC3 | PP10566
Origin Species
Human
NCBI GenBank
NM_017729
SwissProt
Q8TE68

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNRTWPRRIW GSSQDEAELI REDIQGALHN YRSGRGERRA AALRATQEEL QRDRSPAAET
61PPLQRRPSVR AVISTVERGA GRGRPQAKPI PEAEEAQRPE PVGTSSNADS ASPDLGPRGP
121DLAVLQAERE VDILNHVFDD VESFVSRLQK SAEAARVLEH RERGRRSRRR AAGEGLLTLR
181AKPPSEAEYT DVLQKIKYAF SLLARLRGNI ADPSSPELLH FLFGPLQMIV NTSGGPEFAS
241SVRRPHLTSD AVALLRDNVT PRENELWTSL GDSWTRPGLE LSPEEGPPYR PEFFSGWEPP
301VTDPQSRAWE DPVEKQLQHE RRRRQQSAPQ VAVNGHRDLE PESEPQLESE TAGKWVLCNY
361DFQARNSSEL SVKQRDVLEV LDDSRKWWKV RDPAGQEGYV PYNILTPYPG PRLHHSQSPA
421RSLNSTPPPP PAPAPAPPPA LARPRWDRPR WDSCDSLNGL DPSEKEKFSQ MLIVNEELQA
481RLAQGRSGPS RAVPGPRAPE PQLSPGSDAS EVRAWLQAKG FSSGTVDALG VLTGAQLFSL
541QKEELRAVSP EEGARVYSQV TVQRSLLEDK EKVSELEAVM EKQKKKVEGE VEMEVI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

596 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.