Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EPS15L1 / EPS15R Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101743-100
Catalog Number: LS-G101743
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EPS15R(EPS15L1) (NM_021235), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Eps15-related protein | EPS15L1 | EPS15R
- Origin Species
- Human
- NCBI GenBank
- NM_021235
- SwissProt
- Q9UBC2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 94.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPLIPLSQ QIPTGNSLYE SYYKQVDPAY TGRVGASEAA LFLKKSGLSD IILGKIWDLA61DPEGKGFLDK QGFYVALRLV ACAQSGHEVT LSNLNLSMPP PKFHDTSSPL MVTPPSAEAH121WAVRVEEKAK FDGIFESLLP INGLLSGDKV KPVLMNSKLP LDVLGRVWDL SDIDKDGHLD181RDEFAVAMHL VYRALEKEPV PSALPPSLIP PSKRKKTVFP GAVPVLPASP PPKDSLRSTP241SHGSVSSLNS TGSLSPKHSL KQTQPTVNWV VPVADKMRFD EIFLKTDLDL DGYVSGQEVK301EIFMHSGLTQ NLLAHIWALA DTRQTGKLSK DQFALAMYFI QQKVSKGIDP PQVLSPDMVP361PSERGTPGPD SSGSLGSGEF TGVKELDDIS QEIAQLQREK YSLEQDIREK EEAIRQKTSE421VQELQNDLDR ETSSLQELEA QKQDAQDRLD EMDQQKAKLR DMLSDVRQKC QDETQMISSL481KTQIQSQESD LKSQEDDLNR AKSELNRLQQ EETQLEQSIQ AGRVQLETII KSLKSTQDEI541NQARSKLSQL HESRQEAHRS LEQYDQVLDG AHGASLTDLA NLSEGVSLAE RGSFGAMDDP601FKNKALLFSN NTQELHPDPF QTEDPFKSDP FKGADPFKGD PFQNDPFAEQ QTTSTDPFGG661DPFKESDPFR GSATDDFFKK QTKNDPFTSD PFTKNPSLPS KLDPFESSDP FSSSSVSSKG721SDPFGTLDPF GSGSFNSAEG FADFSQMSKP PPSGPFTSSL GGAGFSDDPF KSKQDTPALP781PKKPAPPRPK PPSGKSTPVS QLGSADFPEA PDPFQPLGAD SGDPFQSKKG FGDPFSGKDP841FVPSSAAKPS KASASGFADF TSVSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
864 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.