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EPN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EPN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EPN2 antibodies →
$504.00each
Catalog Number: LS-G118835-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Epsin 2(EPN2) (NM_001102664), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Epsin-2 | EPN2 | EHB21 | EPS-15-interacting protein 2 | Eps15 binding protein | Epsin 2 | KIAA1065
Origin Species
Human
NCBI GenBank
NM_014964
SwissProt
O95208

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.27 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV VAFSEIMSMV
61WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI FAIQTLKDFQ YIDRDGKDQG
121INVREKSKQL VALLKDEERL KAERAQALKT KERMAQVATG MGSNQITFGR GSSQPNLSTS
181HSEQEYGKAG GSPASYHGST SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQEERL
241RRGDDLRLQM ALEESRRDTV KIPKKKEHGS LPQQTTLLDL MDALPSSGPA AQKAEPWGPS
301ASTNQTNPWG GPAAPASTSD PWPSFGTKPA ASIDPWGVPT GATAQSVPKN SDPWAASQQP
361ASSAGKRASD AWGAVSTTKP VSVSGSFELF SNLNGTIKDD FSEFDNLRTS KKTAESVTSL
421PSQNNGTTSP DPFESQPLTV ASSKPSSARK TPESFLGPNA ALVNLDSLVT RPAPPAQSLN
481PFLAPGAPAT SAPVNPFQVN QPQPLTLNQL RGSPVLGTST SFGPGPGVES MAVASMTSAA
541PQPALGATGS SLTPLGPAMM NMVGSVGIPP SAAQATGTTN PFLL
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

584 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.