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EPM2AIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EPM2AIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113681-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EPM2AIP1 (NM_014805), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EPM2A interacting protein 1 | EPM2A-interacting protein 1 | EPM2AIP1 | KIAA0766 | Laforin-interacting protein
Origin Species
Human
NCBI GenBank
NM_014805
SwissProt
Q7L775

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWMTPKRSKM EVDEALVFRP EWTQRYLVVE PPEGDGALCL VCRRLIVATR ERDVRRHYEA
61EHEYYERYVA DGERAALVER LRQGDLPVAS FTPEERAARA GLGLCRLLAL KGRGWGEGDF
121VYQCMEVLLR EVLPEHVSVL QGVDLSPDIT RQRILSIDRN LRNQLFNRAR DFKAYSLALD
181DQAFVAYENY LLVFIRGVGP ELEVQEDLLT IINLTHHFSV GALMSAILES LQTAGLSLQR
241MVGLTTTHTL RMIGENSGLV SYMREKAVSP NCWNVIHYSG FLHLELLSSY DVDVNQIINT
301ISEWIVLIKT RGVRRPEFQT LLTESESEHG ERVNGRCLNN WLRRGKTLKL IFSLRKEMEA
361FLVSVGATTV HFSDKQWLCD FGFLVDIMEH LRELSEELRV SKVFAAAAFD HICTFEVKLN
421LFQRHIEEKN LTDFPALREV VDELKQQNKE DEKIFDPDRY QMVICRLQKE FERHFKDLRF
481IKKDLELFSN PFNFKPEYAP ISVRVELTKL QANTNLWNEY RIKDLGQFYA GLSAESYPII
541KGVACKVASL FDSNQICEKA FSYLTRNQHT LSQPLTDEHL QALFRVATTE MEPGWDDLVR
601ERNESNP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

607 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.