Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EPHX4 / Epoxide Hydrolase 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105214-100
Catalog Number: LS-G105214
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABHD7(EPHX4) (NM_173567), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EPHXRP | ABHD7 | Epoxide hydrolase 4 | EPHX4
- Origin Species
- Human
- NCBI GenBank
- AA947961
- SwissProt
- Q8IUS5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MARLRDCLPR LMLTLRSLLF WSLVYCYCGL CASIHLLKLL WSLGKGPAQT FRRPAREHPP61ACLSDPSLGT HCYVRIKDSG LRFHYVAAGE RGKPLMLLLH GFPEFWYSWR YQLREFKSEY121RVVALDLRGY GETDAPIHRQ NYKLDCLITD IKDILDSLGY SKCVLIGHDW GGMIAWLIAI181CYPEMVMKLI VINFPHPNVF TEYILRHPAQ LLKSSYYYFF QIPWFPEFMF SINDFKVLKH241LFTSHSTGIG RKGCQLTTED LEAYIYVFSQ PGALSGPINH YRNIFSCLPL KHHMVTTPTL301LLWGENDAFM EVEMAEVTKI FVKNYFRLTI LSEASHWLQQ DQPDIVNKLI WTFLKEETRK361KDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
362 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.