Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EPHA6 / EPH Receptor A6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99316-100
Catalog Number: LS-G99316
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Eph receptor A6(EPHA6) (NM_001080448), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EPHA6 | Ephrin receptor EphA6 | EPA6 | EPH-like kinase 12 | EHK2 | Ek1 | Ephrin type-A receptor 6 | EHK-2 | EK12 | EPH homology kinase 2 | EPH receptor A6 | HEK12 | PRO57066
- NCBI GenBank
- AX250164
- SwissProt
- Q9UF33
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 126.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQFPSPPAAR SSPAPQAASS SEAAAPATGQ PGPSCPVPGT SRRGRPGTPP AGRVEEEEEE61EEEDVDKDPH PTQNTCLRCR HFSLRERKRE PRRTMGGCEV REFLLQFGFF LPLLTAWPGD121CSHVSNNQVV LLDTTTVLGE LGWKTYPLNG WDAITEMDEH NRPIHTYQVC NVMEPNQNNW181LRTNWISRDA AQKIYVEMKF TLRDCNSIPW VLGTCKETFN LFYMESDESH GIKFKPNQYT241KIDTIAADES FTQMDLGDRI LKLNTEIREV GPIERKGFYL AFQDIGACIA LVSVRVFYKK301CPFTVRNLAM FPDTIPRVDS SSLVEVRGSC VKSAEERDTP KLYCGADGDW LVPLGRCICS361TGYEEIEGSC HACRPGFYKA FAGNTKCSKC PPHSLTYMEA TSVCQCEKGY FRAEKDPPSM421ACTRPPSAPR NVVFNINETA LILEWSPPSD TGGRKDLTYS VICKKCGLDT SQCEDCGGGL481RFIPRHTGLI NNSVIVLDFV SHVNYTFEIE AMNGVSELSF SPKPFTAITV TTDQDAPSLI541GVVRKDWASQ NSIALSWQAP AFSNGAILDY EIKYYEKEHE QLTYSSTRSK APSVIITGLK601PATKYVFHIR VRTATGYSGY SQKFEFETGD ETSDMAAEQG QILVIATAAV GGFTLLVILT661LFFLITGRCQ WYIKAKMKSE EKRRNHLQNG HLRFPGIKTY IDPDTYEDPS LAVHEFAKEI721DPSRIRIERV IGAGEFGEVC SGRLKTPGKR EIPVAIKTLK GGHMDRQRRD FLREASIMGQ781FDHPNIIRLE GVVTKRSFPA IGVEAFCPSF LRAGFLNSIQ APHPVPGGGS LPPRIPAGRP841VMIVVEYMEN GSLDSFLRKH DGHFTVIQLV GMLRGIASGM KYLSDMGYVH RDLAARNILV901NSNLVCKVSD FGLSRVLEDD PEAAYTTTGG KIPIRWTAPE AIAYRKFSSA SDAWSYGIVM961WEVMSYGERP YWEMSNQDVI LSIEEGYRLP APMGCPASLH QLMLHCWQKE RNHRPKFTDI1021VSFLDKLIRN PSALHTLVED ILVMPESPGE VPEYPLFVTV GDWLDSIKMG QYKNNFVAAG1081FTTFDLISRM SIDDIRRIGV ILIGHQRRIV SSIQTLRLHM MHIQEKGFHVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1130 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.