LSBio
EPB41L4A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EPB41L4A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110432-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EPB41L4A (NM_022140), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Band 4.1-like protein 4A | EPB41L4 | EPB41L4A | Protein NBL4 | NBL4
Origin Species
Human
NCBI GenBank
NM_022140
SwissProt
Q9HCS5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGCFCAVPEE FYCEVLLLDE SKLTLTTQQQ GIKKSTKGSV VLDHVFHHVN LVEIDYFGLR
61YCDRSHQTYW LDPAKTLAEH KELINTGPPY TLYFGIKFYA EDPCKLKEEI TRYQFFLQVK
121QDVLQGRLPC PVNTAAQLGA YAIQSELGDY DPYKHTAGYV SEYRFVPDQK EELEEAIERI
181HKTLMGQIPS EAELNYLRTA KSLEMYGVDL HPVYGENKSE YFLGLTPVGV VVYKNKKQVG
241KYFWPRITKV HFKETQFELR VLGKDCNETS FFFEARSKTA CKHLWKCSVE HHTFFRMPEN
301ESNSLSRKLS KFGSIRYKHR YSGRTALQMS RDLSIQLPRP DQNVTRSRSK TYPKRIAQTQ
361PAESNSISRI TANMENGENE GTIKIIAPSP VKSFKKAKNE NSPDTQRSKS HAPWEENGPQ
421SGLYNSPSDR TKSPKFPYTR RRNPSCGSDN DSVQPVRRRK AHNSGEDSDL KQRRRSRSRC
481NTSSGSESEN SNREYRKKRN RIRQENDMVD SAPQWEAVLR RQKEKNQADP NNRRSRHRSR
541SRSPDIQAKE ELWKHIQKEL VDPSGLSEEQ LKEIPYTKIE TQGDPIRIRH SHSPRSYRQY
601RRSQCSDGER SVLSEVNSKT DLVPPLPVTH SSDAQGSGDA TVHQRRNGSK DSLMEEKPQT
661STNNLAGKHT AKTIKTIQAS RLKTET
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

686 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.