Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EPB41L1 / 4.1N Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102479-100
Catalog Number: LS-G102479
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EPB41L1 (NM_177996), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 4.1N | MRD11 | Band 4.1-like protein 1 | EPB41L1 | KIAA0338 | Neuronal protein 4.1
- Origin Species
- Human
- NCBI GenBank
- AB002336
- SwissProt
- Q9H4G0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEKDYSEAD GLSERTTPSK AQKSPQKIAK KYKSAICRVT LLDASEYECE VEKHGRGQVL61FDLVCEHLNL LEKDYFGLTF CDADSQKNWL DPSKEIKKQI RSSPWNFAFT VKFYPPDPAQ121LTEDITRYYL CLQLRADIIT GRLPCSFVTH ALLGSYAVQA ELGDYDAEEH VGNYVSELRF181APNQTRELEE RIMELHKTYR GMTPGEAEIH FLENAKKLSM YGVDLHHAKD SEGIDIMLGV241CANGLLIYRD RLRINRFAWP KILKISYKRS NFYIKIRPGE YEQFESTIGF KLPNHRSAKR301LWKVCIEHHT FFRLVSPEPP PKGFLVMGSK FRYSGRTQAQ TRQASALIDR PAPFFERSSS361KRYTMSRSLD GAEFSRPASV SENHDAGPDG DKRDEDGESG GQRSEAEEGE VRTPTKIKEL421KFLDKPEDVL LKHQASINEL KRTLKEPNSK LIHRDRDWER ERRLPSSPAS PSPKGTPEKA481NERAGLREGS EEKVKPPRPR APESDTGDED QDQERDTVFL KDNHLAIERK CSSITVSSTS541SLEAEVDFTV IGDYHGSAFE DFSRSLPELD RDKSDSDTEG LLFSRDLNKG APSQDDESGG601IEDSPDRGAC STPDMPQFEP VKTETMTVSS LAIRKKIEPE AVLQTRVSAM DNTQENSLKS661GKGAAAMIPG PQTVATEIRS LSPIIGKDVL TSTYGATAET LSTSTTTHVT KTVKGGFSET721RIEKRIIITG DEDVDQDQAL ALAIKEAKLQ HPDMLVTKAV VYRETDPSPE ERDKKPQEST781RTRPLEQKLI SEEDLAANDI LDYKDDDDKV
810 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.