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ENTPD8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ENTPD8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ENTPD8 antibodies →
$725.00each
Catalog Number: LS-G120914-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ENTPD8 (NM_001033113), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ENTPD8 | NTPDase 8 | NTPDase-8 | NTPDase8 | UNQ2492 | E-NTPDase 8 | Ecto-ATP-diphosphohydrolase | GLSR2492
Origin Species
Human
SwissProt
Q5MY95

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MGLSRKEQVF LALLGASGVS GLTALILLLV EATSVLLPTD IKFGIVFDAG SSHTSLFLYQ
61WLANKENGTG VVSQALACQV EGPGISSYTS NAAQAGESLQ GCLEEALVLI PEAQHRKTPT
121FLGATAGMRL LSRKNSSQAR DIFAAVTQVL GRSPVDFWGA ELLAGQAEGA FGWITVNYGL
181GTLVKYSFTG EWIQPPEEML VGALDMGGAS TQITFVPGGP ILDKSTQADF RLYGSDYSVY
241THSYLCFGRD QMLSRLLVGL VQSRPAALLR HPCYLSGYQT TLALGPLYES PCVHATPPLS
301LPQNLTVEGT GNPGACVSAI RELFNFSSCQ GQEDCAFDGV YQPPLRGQFY AFSNFYYTFH
361FLNLTSRQPL STVNATIWEF CQRPWKLVEA SYPGQDRWLR DYCASGLYIL TLLHEGYGFS
421EETWPSLEFR KQAGGVDIGW TLGYMLNLTG MIPADAPAQW RAESYGVWVA KVVFMVLALV
481AVVGAALVQL FWLQD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

495 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.