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ENTPD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ENTPD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ENTPD7 antibodies →
$725.00each
Catalog Number: LS-G111207-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ENTPD7 (NM_020354), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ENTPD7 | NTPDase 7 | RP11-483F11.1 | LALP1
Origin Species
Human
NCBI GenBank
NM_020354
SwissProt
Q9NQZ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARISFSYLC PASWYFTVPT VSPFLRQRVA FLGLFFISCL LLLMLIIDFR HWSASLPRDR
61QYERYLARVG ELEATDTEDP NLNYGLVVDC GSSGSRIFVY FWPRHNGNPH DLLDIKQMRD
121RNSQPVVKKI KPGISAMADT PEHASDYLRP LLSFAAAHVP VKKHKETPLY ILCTAGMRLL
181PERKQLAILA DLVKDLPLEF DFLFSQSQAE VISGKQEGVY AWIGINFVLG RFDHEDESDA
241EATQELAAGR RRTVGILDMG GASLQIAYEV PTSTSVLPAK QEEAAKILLA EFNLGCDVQH
301TEHVYRVYVT TFLGFGGNFA RQRYEDLVLN ETLNKNRLLG QKTGLSPDNP FLDPCLPVGL
361TDVVERNSQV LHVRGRGDWV SCGAMLSPLL ARSNTSQASL NGIYQSPIDF NNSEFYGFSE
421FFYCTEDVLR IGGRYHGPTF AKAAQDYCGM AWSVLTQRFK NGLFSSHADE HRLKYQCFKS
481AWMYQVLHEG FHFPYDYPNL RTAQLVYDRE VQWTLGAILY KTRFLPLRDL RQEGVRQAHG
541SWFRLSFVYN HYLFFACILV VLLAIFLYLL RLRRIHHRQT RASAPLDLLW LEEVVPMMGV
601QVGP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

604 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.