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ENTPD6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ENTPD6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ENTPD6 antibodies →
$504.00each
Catalog Number: LS-G99381-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ENTPD6 (NM_001247), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CD39-like 2 | CD39L2 | DJ738P15.3 | ENTPD6 | HCV-E2 binding protein 1 | IL-6SAG | NTPDase-6 | IL6ST2 | CD39 antigen-like 2 | NTPDase 6
NCBI GenBank
NM_001247
SwissProt
O75354

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MKKGIRYETS RKTSYIFQPQ HGPWQTRMRK ISNHGSLRVA KVAYPLGLCV GVFIYVAYIK
61WHRATATQAF FSITRAAPGA RWGQQAHSPL GTAADGHEVF YGIMFDAGST GTRVHVFQFT
121RPPRETPTLT HETFKALKPG LSAYADDVEK SAQGIRELLD VAKQDIPFDF WKATPLVLKA
181TAGLRLLPGE KAQKLLQKVK EVFKASPFLV GDDCVSIMNG TDEGVSAWIT INFLTGSLKT
241PGGSSVGMLD LGGGSTQIAF LPRVEGTLQA SPPGYLTALR MFNRTYKLYS YSYLGLGLMS
301ARLAILGGVE GQPAKDGKEL VSPCLSPSFK GEWEHAEVTY RVSGQKAAAS LHELCAARVS
361EVLQNRVHRT EEVKHVDFYA FSYYYDLAAG VGLIDAEKGG SLVVGDFEIA AKYVCRTLET
421QPQSSPFSCM DLTYVSLLLQ EFGFPRSKVL KLTRKIDNVE TSWALGAIFH YIDSLNRQKS
481PAS
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

483 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.