Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ENTPD4 / LALP70 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124880-100
Catalog Number: LS-G124880
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ENTPD4 (NM_001128930), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ENTPD4 | KIAA0392 | LALP70 | LAP70 | LYSAL1 | NTPDase-4 | Lysosomal apyrase-like 1 | Uridine-diphosphatase | Golgi luminal UDPase | NTPDase 4 | UDPase
- Origin Species
- Human
- NCBI GenBank
- NM_004901
- SwissProt
- Q9Y227
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRIGISCLF PASWHFSISP VGCPRILNTN LRQIMVISVL AAAVSLLYFS VVIIRNKYGR61LTRDKKFQRY LARVTDIEAT DTNNPNVNYG IVVDCGSSGS RVFVYCWPRH NGNPHDLLDI121RQMRDKNRKP VVMKIKPGIS EFATSPEKVS DYISPLLNFA AEHVPRAKHK ETPLYILCTA181GMRILPESQQ KAILEDLLTD IPVHFDFLFS DSHAEVISGK QEGVYAWIGI NFVLGRFEHI241EDDDEAVVEV NIPGSESSEA IVRKRTAGIL DMGGVSTQIA YEVPKTEEVA KNLLAEFNLG301CDVHQTEHVY RVYVATFLGF GGNAARQRYE DRIFANTIQK NRLLGKQTGL TPDMPYLDPC361LPLDIKDEIQ QNGQTIYLRG TGDFDLCRET IQPFMNKTNE TQTSLNGVYQ PPIHFQNSEF421YGFSEFYYCT EDVLRMGGDY NAAKFTKAAK DYCATKWSIL RERFDRGLYA SHADLHRLKY481QCFKSAWMFE VFHRGFSFPV NYKSLKTALQ VYDKEVQWTL GAILYRTRFL PLRDIQQEAF541RASHTHWRGV SFVYNHYLFS GCFLVVLLAI LLYLLRLRRI HRRTPRSSSA AALWMEEGLP601AQNAPGTLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
608 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.