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ENTHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ENTHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ENTHD1 antibodies →
$504.00each
Catalog Number: LS-G106237-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ENTHD1 (NM_152512), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ENTH domain containing 1 | Epsin-2B | ENTHD1 | DJ370M22.3 | DJ370M22.3 (EPSIN 2B)
Origin Species
Human
SwissProt
Q8IYW4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAFRRQVKNF VKNYSDAEIK VREATSNDPW GPSSSLMLDI SDLTFNTISL SEIMNMLWHR
61LNDHGKNWRH VYKSLTLMDY LIKNGSKKVI QHCREGFCNL QTLKDFQHID EAGKDQGYYI
121REKSKQVITL LMDEPLLCKE REVACRTRQR TSHSILFSKR QLGSSNSLTA CTSAPTPDIS
181ASEKKYKLPK FGRLHNKRNV CKAGLKQEHC QDVHLPTETM LSQETLPLKI HGWKSTEDLM
241TFLDDDPELP LLATPPSIVS PITCLSEAEE VCNLSGADAV PTLSENSPSG QRDVSLDKRS
301DGIFTNTVTE NLLETPLEKQ SAAEGLKTLT ILPACWSSKE EFISPDLRVS KSDSTFHNQA
361SVETLCLSPS FKIFDRVKEI VINKAYQKPA QSSIQMDDKI LKTTTRVSTA SEGASSFSPL
421SMSSPDLASP EKSAHLLSPI LAGPSFWTLS HQQLSSTSFK DEDKTAKLHH SFASRGPVSS
481DVEENDSLNL LGILPNNSDS AKKNISHISS SHWGEFSTQN VDQFIPLSCS GFQSTKDFPQ
541EPEAKNSISV LLREVKRAIA RLHEDLSTVI QELNVINNIL MSMSLNSSQI SQSSQVPQSS
601EGSSDQI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

607 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.