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ENOX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ENOX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ENOX2 antibodies →
$725.00each
Catalog Number: LS-G115347-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ENOX2 (NM_006375), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
APK1 antigen | APK1 | TNOX | COVA1 | ENOX2
Origin Species
Human
NCBI GenBank
NM_006375
SwissProt
Q16206

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTLPMSDPTA WATAMNNLGM APLGIAGQPI LPDFDPALGM MTGIPPITPM MPGLGIVPPP
61IPPDMPVVKE IIHCKSCTLF PPNPNLPPPA TRERPPGCKT VFVGGLPENG TEQIIVEVFE
121QCGEIIAIRK SKKNFCHIRF AEEYMVDKAL YLSGYRIRLG SSTDKKDTGR LHVDFAQARD
181DLYEWECKQR MLAREERHRR RMEEERLRPP SPPPVVHYSD HECSIVAEKL KDDSKFSEAV
241QTLLTWIERG EVNRRSANNF YSMIQSANSH VRRLVNEKAA HEKDMEEAKE KFKQALSGIL
301IQFEQIVAVY HSASKQKAWD HFTKAQRKNI SVWCKQAEEI RNIHNDELMG IRREEEMEMS
361DDEIEEMTET KETEESALVS QAEALKEEND SLRWQLDAYR NEVELLKQEQ GKVHREDDPN
421KEQQLKLLQQ ALQGMQQHLL KVQEEYKKKE AELEKLKDDK LQVEKMLENL KEKESCASRL
481CASNQDSEYP LEKTMNSSPI KSEREALLVG IISTFLHVHP FGASIEYICS YLHRLDNKIC
541TSDVECLMGR LQHTFKQEMT GVGASLEKRW KFCGFEGLKL T
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

581 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.