Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ENOX1 / CNOX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101520-100
Catalog Number: LS-G101520
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ENOX1 (NM_017993), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCNOX | CNOX | ENOX1 | PIG38 | BA64J21.1 | Constitutive Ecto-NOX
- Origin Species
- Human
- NCBI GenBank
- NM_017993
- SwissProt
- Q8TC92
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVDAGGVENI TQLPQELPQM MAAAADGLGS IAIDTTQLNM SVTDPTAWAT AMNNLGMVPV61GLPGQQLVSD SICVPGFDPS LNMMTGITPI NPMIPGLGLV PPPPPTEVAV VKEIIHCKSC121TLFPQNPNLP PPSTRERPPG CKTVFVGGLP ENATEEIIQE VFEQCGDITA IRKSKKNFCH181IRFAEEFMVD KAIYLSGYRM RLGSSTDKKD SGRLHVDFAQ ARDDFYEWEC KQRMRAREER241HRRKLEEDRL RPPSPPAIMH YSEHEAALLA EKLKDDSKFS EAITVLLSWI ERGEVNRRSA301NQFYSMVQSA NSHVRRLMNE KATHEQEMEE AKENFKNALT GILTQFEQIV AVFNASTRQK361AWDHFSKAQR KNIDIWRKHS EELRNAQSEQ LMGIRREEEM EMSDDENCDS PTKKMRVDES421ALAAQAYALK EENDSLRWQL DAYRNEVELL KQEKEQLFRT EENLTKDQQL QFLQQTMQGM481QQQLLTIQEE LNNKKSELEQ AKEEQSHTQA LLKVLQEQLK GTKELVETNG HSHEDSNEIN541VLTVALVNQD RENNIEKRSQ GLKSEKEALL IGIISTFLHV HPFGANIEYL WSYMQQLDSK601ISANEIEMLL MRLPRMFKQE FTGVGATLEK RWKLCAFEGI KTTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
643 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.