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EML5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EML5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EML5 antibodies →
$725.00each
Catalog Number: LS-G104029-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EML5 (NM_183387), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EMAP-5 | EML5 | EMAP-2 | HuEMAP-2
Origin Species
Human
NCBI GenBank
NM_183387
SwissProt
Q05BV3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
220.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAARSAPSCH LRLEWVYGYR GHQCRNNLYY TAAKEIVYFV AGVGVVYSPR EHRQKFYRGH
61SDDIISLALH PERVLVATGQ VGKEPYICIW DSYTVQTISV LKDVHTHGIA CLAFDLDGQR
121LVSVGLDSKN AVCVWDWKRG KMLSMAPGHT DRIFDISWDL YQPNKLVSCG VKHIKFWSLC
181GNALTPKRGV FGKTGDLQTI LCLACARDEL TYSGALNGDI YVWKGINLIR TIQGAHAAGI
241FSMNACEEGF ATGGRDGCIR LWDLTFKPIT VIDLRETDQG YKGLSVRSVC WRGDHILVGT
301QDSEIFEIVV QERNKPFLIM QGHCEGELWA LAVHPTKPLA VTGSDDRSVR IWSLVDHALI
361ARCNMEEPIR CAAVNADGIH LALGMKDGSF TVLRVRDMTE VVHIKDRKEA IHELKYSPDG
421TYLAVGCNDS SVDIYGVAQR YKKVGECLGS LSFITHLDWS SDSRYLQTND GNGKRLFYRM
481PGGKEVTSTE EIKGVHWASW TCVSGLEVNG IWPKYSDIND INSVDGNYIG QVLVTADDYG
541IIKLFRYPCL RKGAKFRKYI GHSAHVTNVR WSHDYQWVIS IGGADHSVFQ WKFIPERKLK
601DAVHIAPQES LADSHSDESD SDLSDVPELD SEIEQETQLT YRRQVYKEDL PQLKEQCKEK
661QKSATSKRRE RAPGNSIRLH FVHGYRGYDC RSNLFYTQIG EIVYHVAAVG VIYNRQQNTQ
721RFYLGHDDDI LCLTIHPLKD YVATGQVGRD PSIHIWDTET IKPLSILKGH HQYGVSAVDF
781SADGKRLASV GIDDSHTVVL WDWKKGEKLS IARGSKDKIF VVKMNPYVPD KLITAGIKHM
841KFWRKAGGGL IGRKGYIGTL GKNDTMMCAV YGWTEEMAFS GTSTGDVCIW RDIFLVKTVK
901AHDGPVFSMH ALEKGFVTGG KDGIVALWDD SFERCLKTYA IKRAALAPGS KGLLLEDNPS
961IRAISLGHGH ILVGTKNGEI LEVDKSGPIT LLVQGHMEGE VWGLATHPYL PICATVSDDK
1021TLRIWDLSPS HCMLAVRKLK KGGRCCCFSP DGKALAVGLN DGSFLMANAD TLEDLVSFHH
1081RKDMISDIRF SPGSGKYLAV ASHDSFIDIY NVMSSKRVGI CKGATSYITH IDWDIRGKLL
1141QVNTGAKEQL FFEAPRGKKQ TIPSVEVEKI AWASWTSVLG LCCEGIWPVI GEVTDVTASC
1201LTSDKMVLAT GDDLGFVKLF RYPTKGKFGK FKRYVAHSTH VTNVRWTYDD SMLVTLGGTD
1261MSLMVWTNEM EGYREKRPCD SEESDIDSEE DGGYDSDVTR ENEISYTIRA LSTNIRPMLG
1321IKPHLQQKEP SIDERQGVVR GSRPPVSRAP PQPEKLQTNN VGKKKRPIED LVLELIFGYR
1381GRDCRNNVHY LNDGDDIIYH TASVGILHNV ATGSQSFYQE HNDDILCLTV NQHPKFINIV
1441ATGQVGDSAD MSATAPSIHI WDAMNKQTLS ILRCYHSKGV CSVSFSATGK LLLSVGLDPE
1501HTITIWRWQE GAKIASRAGH NQRIFVAEFR PDSDTQFVSV GVKHVKFWTL AGRALLSKKG
1561LLSTLEDARM QTMLAIAFGA NNLTFTGTIS GDVCVWKDHI LCRIVARAHN GPVFAMYTTL
1621RDGLIVTGGK ERPSKEGGAV KLWDQELRRC RAFRLETGQA TDCVRSVCRG KGKILVGTRN
1681AEIIEVGEKN AACNILVNGH VDGPIWGLAT HPSRDFFLSA AEDGTVRLWD IADKKMLNKV
1741NLGHAARTVC YSPEGDMVAI GMKNGEFIIL LVSSLKIWGK KRDRRCAIHD IRFSPDSRYL
1801AVGSSENSVD FYDLTLGPTL NRISYCKDIP SFVIQMDFSA DSSYLQVSSG CYKRHVYEVP
1861SGKHLMDHAA IDRITWATWT SILGDEVLGI WSRHAEKADV NCACVSHSGI SLVTGDDFGM
1921VKLFDFPCPE KFAKHKRFLG HSPHVTNIRF TSGDRHVVSA GGDDCSLFVW KCVHTPH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1977 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.