LSBio
EML4 Protein (Over-Expression Lysate Myc-DDK (Flag))

EML4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111458-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EML4 (NM_019063), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2orf2 | EMAP-4 | EML4 | EMAPL4 | Ropp 120 | ELP120 | ROPP120
Origin Species
Human
NCBI GenBank
NM_019063
SwissProt
Q9HC35

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
108.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDGFAGSLDD SISAASTSDV QDRLSALESR VQQQEDEITV LKAALADVLR RLAISEDHVA
61SVKKSVSSKG QPSPRAVIPM SCITNGSGAN RKPSHTSAVS IAGKETLSSA AKSGTEKKKE
121KPQGQREKKE ESHSNDQSPQ IRASPSPQPS SQPLQIHRQT PESKNATPTK SIKRPSPAEK
181SHNSWENSDD SRNKLSKIPS TPKLIPKVTK TADKHKDVII NQEGEYIKMF MRGRPITMFI
241PSDVDNYDDI RTELPPEKLK LEWAYGYRGK DCRANVYLLP TGEIVYFIAS VVVLFNYEER
301TQRHYLGHTD CVKCLAIHPD KIRIATGQIA GVDKDGRPLQ PHVRVWDSVT LSTLQIIGLG
361TFERGVGCLD FSKADSGVHL CVIDDSNEHM LTVWDWQKKA KGAEIKTTNE VVLAVEFHPT
421DANTIITCGK SHIFFWTWSG NSLTRKQGIF GKYEKPKFVQ CLAFLGNGDV LTGDSGGVML
481IWSKTTVEPT PGKGPKGVYQ ISKQIKAHDG SVFTLCQMRN GMLLTGGGKD RKIILWDHDL
541NPEREIEVPD QYGTIRAVAE GKADQFLVGT SRNFILRGTF NDGFQIEVQG HTDELWGLAT
601HPFKDLLLTC AQDRQVCLWN SMEHRLEWTR LVDEPGHCAD FHPSGTVVAI GTHSGRWFVL
661DAETRDLVSI HTDGNEQLSV MRYSIDGTFL AVGSHDNFIY LYVVSENGRK YSRYGRCTGH
721SSYITHLDWS PDNKYIMSNS GDYEILYWDI PNGCKLIRNR SDCKDIDWTT YTCVLGFQVF
781GVWPEGSDGT DINALVRSHN RKVIAVADDF CKVHLFQYPC SKAKAPSHKY SAHSSHVTNV
841SFTHNDSHLI STGGKDMSII QWKLVEKLSL PQNETVADTT LTKAPVSSTE SVIQSNTPTP
901PPSQPLNETA EEESRISSSP TLLENSLEQT VEPSEDHSEE ESEEGSGDLG EPLYEEPCNE
961ISKEQAKATL LEDQQDPSPS S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

981 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.