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EMILIN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EMILIN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EMILIN3 antibodies →
$725.00each
Catalog Number: LS-G108157-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EMILIN3 (NM_052846), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf130 | DJ620E11.4 | EMILIN3 | EMILIN-3 | EMILIN-5 | EMILIN5
Origin Species
Human
SwissProt
Q9NT22

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MGRRRLLVWL CAVAALLSGA QARGTPLLAR PAPPGASRYS LYTTGWRPRL RPGPHKALCA
61YVVHRNVTCI LQEGAESYVK AEYRQCRWGP KCPGTVTYRT VLRPKYKVGY KTVTDLAWRC
121CPGFTGKRCP EHLTDHGAAS PQLEPEPQIP SGQLDPGPRP PSYSRAAPSP HGRKGPGLFG
181ERLERLEGDV QRLAQTYGTL SGLVASHEDP NRMTGGPRAP AVPVGFGVIP EGLVGPGDRA
241RGPLTPPLDE ILSKVTEVSN TLQTKVQLLD KVHGLALGHE AHLQRLREAP PSPLTSLALL
301EEYVDRRLHR LWGSLLDGFE QKLQGVQSEC DLRVQEVRRQ CEEGQAASRR LHQSLDGREL
361ALRQELSQLG SQLQGLSVSG RGSCCGQLAL INARMDGLER ALQAVTETQR GPGAPAGDEL
421TRLSAAMLEG GVDGLLEGLE TLNGTEGGAR GCCLRLDMGG WGVGGFGTML EERVQSLEER
481LATLAGELSH DSASPGRSAR PLVQTELAVL EQRLVSLETS CTPSTTSAIL DSLVAEVKAW
541QSRSEALLRQ VASHAALLQQ LNGTVAEVQG QLAEGTGSSL QGEITLLKVN LNSVSKSLTG
601LSDSVSQYSD AFLAANTSLD ERERKVEAEV QAIQEQVSSQ GSRLQAGHRQ VLNLRGELEQ
661LKAGVAKVAS GLSRCQDTAQ KLQHTVGHFD QRVAQVEGAC RRLGLLAAGL DSLPTEPLRP
721REGLWSHVDQ LNRTLAQHTQ DIARLRDDLL DCQAQLAEQV RPGQAN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

766 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.