LSBio
EMILIN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EMILIN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EMILIN2 antibodies →
$725.00each
Catalog Number: LS-G109056-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EMILIN2 (NM_032048), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EMILIN-2 | EMILIN2 | FOAP-10 | Protein FOAP-10
Origin Species
Human
NCBI GenBank
NM_032048
SwissProt
Q9BXX0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
115.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWQPRRPWPR VPWRWALALL ALVGAGLCHA GPQPGYPARP SARNKNWCAY IVNKNVSCSV
61LEGSESFIQA QYNCAWNQMP CPSALVYRVN FRPRYVTRYK TVTQLEWRCC PGFRGGDCQE
121GPKDPVKTLR PTPARPRNSL KKATDNEPSQ FSEPRKTLSP TGTAQPSWGV DPKEGPQELQ
181EKKIQVLEEK VLRLTRTVLD LQSSLAGVSE NLKHATQDDA SRTRAPGLSS QHPKPDTTVS
241GDTETGQSPG VFNTKESGMK DIKSELAEVK DTLKNKSDKL EELDGKVKGY EGQLRQLQEA
301AQGPTVTMTT NELYQAYVDS KIDALREELM EGMDRKLADL KNSCEYKLTG LQQQCDDYGS
361SYLGVIELIG EKETSLRKEI NNLRARLQEP SAQANCCDSE KNGDIGQQIK TLDQKIERVA
421EATRMLNGRL DNEFDRLIVP EPDVDFDAKW NELDARINVT EKNAEEHCFY IEETLRGAIN
481GEVGDLKQLV DQKIQSLEDR LGSVLLQMTN NTGAELSPPG AAALPGVSGS GDERVMMELN
541HLKDKVQVVE DICLLNIQGK PHGMEGALPN REDRAVRDSL HLLKSLNDTM HRKFQETEQT
601IQKLQQDFSF LYSQLNHTEN DVTHLQKEMS NCRAGENAGM GRFTKVGEQE RTVDTLPSPQ
661HPVAHCCSQL EERWQRLQSQ VISELDACKE CTQGVQREVS MVEGRVSHME KTCSKLDSIS
721GNLQRIKEGL NKHVSSLWNC VRQMNGTLRS HSRDISGLKN SVQQFYSHVF QISTDLQDLV
781KFQPSAKAPS PPPPAEAPKE PLQPEPAPPR PSGPATAEDP GRRPVLPQRP PEERPPQPPG
841STGVIAETGQ AGPPAGAGVS GRGLPRGVDG QTGSGTVPGA EGFAGAPGYP KSPPVASPGA
901PVPSLVSFSA GLTQKPFPSD GGVVLFNKVL VNDGDVYNPS TGVFTAPYDG RYLITATLTP
961ERDAYVEAVL SVSNASVAQL HTAGYRREFL EYHRPPGALH TCGGPGAFHL IVHLKAGDAV
1021NVVVTGGKLA HTDFDEMYST FSGVFLYPFL SHL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1053 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.