Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EMC2 / TTC35 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113793-100
Catalog Number: LS-G113793
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC35(EMC2) (NM_014673), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0103 | TPR repeat protein 35 | EMC2 | TTC35
- Origin Species
- Human
- NCBI GenBank
- NM_014673
- SwissProt
- Q15006
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAKVSELYDV TWEEMRDKMR KWREENSRNS EQIVEVGEEL INEYASKLGD DIWIIYEQVM61IAALDYGRDD LALFCLQELR RQFPGSHRVK RLTGMRFEAM ERYDDAIQLY DRILQEDPTN121TAARKRKIAI RKAQGKNVEA IRELNEYLEQ FVGDQEAWHE LAELYINEHD YAKAAFCLEE181LMMTNPHNHL YCQQYAEVKY TQGGLENLEL SRKYFAQALK LNNRNMRALF GLYMSASHIA241SNPKASAKTK KDNMKYASWA ASQINRAYQF AGRSKKETKY SLKAVEDMLE TLQITQSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
297 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.