Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EMA / MUC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121231-100
Catalog Number: LS-G121231
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EMA(MUC1) (NM_001018017), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CA15-3 | CD227 | Carcinoma-associated mucin | CD227 antigen | DF3 antigen | Episialin | Epithelial membrane antigen | H23 antigen | H23AG | Krebs von den Lungen-6 | MAM6 | MUC1/ZD | MUC-1/SEC | Mucin-1 | Pem | KL-6 | MUC-1/X | MUC1 | Tumor-associated mucin | Peanut-reactive urinary mucin | Polymorphic epithelial mucin | PUM | EMA | MUC-1 | Mucin 1 | transmembrane
- Origin Species
- Human
- NCBI GenBank
- NM_002456
- SwissProt
- P15941
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 27.57 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTPGTQSPFF LLLLLTVLTV VTGSGHASST PGGEKETSAT QRSSVPSSTE KNAFNSSLED61PSTDYYQELQ RDISEMFLQI YKQGGFLGLS NIKFRPGSVV VQLTLAFREG TINVHDVETQ121FNQYKTEAAS RYNLTISDVS VSDVPFPFSA QSGAGVPGWG IALLVLVCVL VALAIVYLIA181LAVCQCRRKN YGQLDIFPAR DTYHPMSEYP TYHTHGRYVP PSSTDRSPYE KVSAGNGGSS241LSYTNPAVAA TSANLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
255 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.