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ELP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ELP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111487-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ELP4 (NM_019040), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C11orf19 | DJ68P15A.1 | Elongation protein 4 homolog | Elongator complex protein 4 | PAX6 neighbor gene protein | PAX6NEB | PAXNEB | ELP4 | HELP4
Origin Species
Human
NCBI GenBank
NM_019040
SwissProt
Q96EB1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAVATCGSV AASTGSAVAT ASKSNVTSFQ RRGPRASVTN DSGPRLVSIA GTRPSVRNGQ
61LLVSTGLPAL DQLLGGGLAV GTVLLIEEDK YNIYSPLLFK YFLAEGIVNG HTLLVASAKE
121DPANILQELP APLLDDKCKK EFDEDVYNHK TPESNIKMKI AWRYQLLPKM EIGPVSSSRF
181GHYYDASKRM PQELIEASNW HGFFLPEKIS STLKVEPCSL TPGYTKLLQF IQNIIYEEGF
241DGSNPQKKQR NILRIGIQNL GSPLWGDDIC CAENGGNSHS LTKFLYVLRG LLRTSLSACI
301ITMPTHLIQN KAIIARVTTL SDVVVGLESF IGSERETNPL YKDYHGLIHI RQIPRLNNLI
361CDESDVKDLA FKLKRKLFTI ERLHLPPDLS DTVSRSSKMD LAESAKRLGP GCGMMAGGKK
421HLDF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

424 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.