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ELMOD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ELMOD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108969-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBED1(ELMOD3) (NM_032213), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RBED1 | RBM29 | RNA-binding motif protein 29 | RNA binding motif protein 29 | LST3 | RNA-binding protein 29 | ELMOD3
Origin Species
Human
NCBI GenBank
NM_032213
SwissProt
Q96FG2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
44.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNEKSCSFHS KEELRDGQGE RLSAGYSPSY DKDKSVLAFR GIPISELKNH GILQALTTEA
61YEWEPRVVST EVVRAQEEWE AVDTIQPETG SQASSEQPGQ LISFSEALQH FQTVDLSPFK
121KRIQPTIRRT GLAALRHYLF GPPKLHQRLR EERDLVLTIA QCGLDSQDPV HGRVLQTIYK
181KLTGSKFDCA LHGNHWEDLG FQGANPATDL RGAGFLALLH LLYLVMDSKT LPMAQEIFRL
241SRHHIQQFPF CLMSVNITHI AIQALREECL SRECNRQQKV IPVVNSFYAA TFLHLAHVWR
301TQRKTISDSG FVLKGVLFLL GRPRLNAQCP RSREPKVVAR LVLAAVLPHP HFLKFQLTKI
361SITHPLESAS SPFSALTVAL FWSYTYDKHI F
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

391 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.