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ELL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ELL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101024-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ELL2 (NM_012081), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ELL2
Origin Species
Human
NCBI GenBank
NM_012081
SwissProt
O00472

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGGTGGLR EEQRYGLSCG RLGQDNITVL HVKLTETAIR ALETYQSHKN LIPFRPSIQF
61QGLHGLVKIP KNDPLNEVHN FNFYLSNVGK DNPQGSFDCI QQTFSSSGAS QLNCLGFIQD
121KITVCATNDS YQMTRERMTQ AEEESRNRST KVIKPGGPYV GKRVQIRKAP QAVSDTVPER
181KRSTPMNPAN TIRKTHSSST ISQRPYRDRV IHLLALKAYK KPELLARLQK DGVNQKDKNS
241LGAILQQVAN LNSKDLSYTL KDYVFKELQR DWPGYSEIDR RSLESVLSRK LNPSQNATGT
301SRSESPVCSS RDAVSSPQKR LLDSEFIDPL MNKKARISHL TNRVPPTLNG HLNPTSEKSA
361AGLPLPPAAA AIPTPPPLPS TYLPISHPPQ IVNSNSNSPS TPEGRGTQDL PVDSFSQNDS
421IYEDQQDKYT SRTSLETLPP GSVLLKCPKP MEENHSMSHK KSKKKSKKHK EKDQIKKHDI
481ETIEEKEEDL KREEEIAKLN NSSPNSSGGV KEDCTASMEP SAIELPDYLI KYIAIVSYEQ
541RQNYKDDFNA EYDEYRALHA RMETVARRFI KLDAQRKRLS PGSKEYQNVH EEVLQEYQKI
601KQSSPNYHEE KYRCEYLHNK LAHIKRLIGE FDQQQAESWS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

640 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.