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ELFN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ELFN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ELFN2 antibodies →
$725.00each
Catalog Number: LS-G108105-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC62(ELFN2) (NM_052906), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ELFN2 | PPP1R29 | DJ63G5.3 | KIAA1904 | LRRC62
Origin Species
Human
SwissProt
Q5R3F8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLRLGLCAAA LLCVCRPGAV RADCWLIEGD KGYVWLAICS QNQPPYETIP QHINSTVHDL
61RLNENKLKAV LYSSLNRFGN LTDLNLTKNE ISYIEDGAFL GQSSLQVLQL GYNKLSNLTE
121GMLRGMSRLQ FLFVQHNLIE VVTPTAFSEC PSLISIDLSS NRLSRLDGAT FASLASLMVC
181ELAGNPFNCE CDLFGFLAWL VVFNNVTKNY DRLQCESPRE FAGYPLLVPR PYHSLNAIIV
241LQAKCRNGSL PARPVSHPTP YSTDAQREPD ENSGFNPDEI LSVEPPASST TDASAGPAIK
301LHHVTFTSAT LVVIIPHPYS KMYILVQYNN SYFSDVMTLK NKKEIVTLDK LRAHTEYTFC
361VTSLRNSRRF NHTCLTFTTR DPVPGDLAPS TSTTTHYIMT ILGCLFGMVI VLGAVYYCLR
421KRRMQEEKQK SVNVKKTILE MRYGADVDAG SIVHAAQKLG EPPVLPVSRM ASIPSMIGEK
481LPTAKGLEAG LDTPKVATKG NYIEVRTGAG GDGLARPEDD LPDLENGQGS AAEISTIAKE
541VDKVNQIINN CIDALKLDSA SFLGGGSSSG DPELAFECQS LPAAAAASSA TGPGALERPS
601FLSPSYKESS HHPLQRQLSA DAAVTRKTCS VSSSGSIKSA KVFSLDVPDH PAATGLAKGD
661SKYIEKGSPL NSPLDRLPLV PAGSGGGSGG GGGIHHLEVK PAYHCSEHRH SFPALYYEEG
721ADSLSQRVSF LKPLTRSKRD STYSQLSPRH YYSGYSSSPE YSSESTHKIW ERFRPYKKHH
781REEVYMAAGH ALRKKVQFAK DEDLHDILDY WKGVSAQQKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

820 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.