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ELFN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ELFN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ELFN1 antibodies →
$725.00each
Catalog Number: LS-G124838-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ELFN1 (NM_001128636), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ELFN1 | PPP1R28
Origin Species
Human
NCBI GenBank
NM_001128636
SwissProt
P0C7U0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGRGWGALW VCVAAATLLH AGGLARADCW LIEGDKGFVW LAICSQNQPP YEAIPQQINS
61TIVDLRLNEN RIRSVQYASL SRFGNLTYLN LTKNEIGYIE DGAFSGQFNL QVLQLGYNRL
121RNLTEGMLRG LGKLEYLYLQ ANLIEVVMAS SFWECPNIVN IDLSMNRIQQ LNSGTFAGLA
181KLSVCELYSN PFYCSCELLG FLRWLAAFTN ATQTYDRMQC ESPPVYSGYY LLGQGRRGHR
241SILSKLQSVC TEDSYAAEVV GPPRPASGRS QPGRSPPPPP PPEPSDMPCA DDECFSGDGT
301TPLVALPTLA TQAEARPLIK VKQLTQNSAT ITVQLPSPFH RMYTLEHFNN SKASTVSRLT
361KAQEEIRLTN LFTLTNYTYC VVSTSAGLRH NHTCLTICLP RLPSPPGPVP SPSTATHYIM
421TILGCLFGMV LVLGAVYYCL RRRRRQEEKH KKAASAAAAG SLKKTIIELK YGPELEAPGL
481APLSQGPLLG PEAVTRIPYL PAAGEVEQYK LVESADTPKA SKGSYMEVRT GDPPERRDCE
541LGRPGPDSQS SVAEISTIAK EVDKVNQIIN NCIDALKSES TSFQGVKSGP VSVAEPPLVL
601LSEPLAAKHG FLAPGYKDAF GHSLQRHHSV EAAGPPRAST SSSGSVRSPR AFRAEAVGVH
661KAAAAEAKYI EKGSPAADAI LTVTPAAAVL RAEAEKGRQY GEHRHSYPGS HPAEPPAPPG
721PPPPPPHEGL GRKASILEPL TRPRPRDLAY SQLSPQYHSL SYSSSPEYTC RASQSIWERF
781RLSRRRHKEE EEFMAAGHAL RKKVQFAKDE DLHDILDYWK GVSAQHKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

828 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.