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ELAC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ELAC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101591-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ELAC1 (NM_018696), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
D29 | Deleted in Ma29 | ElaC (E. coli) homolog 1 | ElaC homolog 1 (E. coli) | TRNA 3 endonuclease 1 | TRNA Z (short form) | Ribonuclease Z 1 | TRNase Z 1 | ElaC homolog protein 1 | ELAC1 | RNase Z 1 | TRNase ZS
Origin Species
Human
NCBI GenBank
NM_018696
SwissProt
Q9H777

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
39.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MSMDVTFLGT GAAYPSPTRG ASAVVLRCEG ECWLFDCGEG TQTQLMKSQL KAGRITKIFI
61THLHGDHFFG LPGLLCTISL QSGSMVSKQP IEIYGPVGLR DFIWRTMELS HTELVFHYVV
121HELVPTADQC PAEELKEFAH VNRADSPPKE EQGRTILLDS EENSYLLFDD EQFVVKAFRL
181FHRIPSFGFS VVEKKRPGKL NAQKLKDLGV PPGPAYGKLK NGISVVLENG VTISPQDVLK
241KPIVGRKICI LGDCSGVVGD GGVKLCFEAD LLIHEATLDD AQMDKAKEHG HSTPQMAATF
301AKLCRAKRLV LTHFSQRYKP VALAREGETD GIAELKKQAE SVLDLQEVTL AEDFMVISIP
361IKKTRTRPLE QKLISEEDLA ANDILDYKDD DDKV

394 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.