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EIF4G3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EIF4G3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EIF4G3 antibodies →
$725.00each
Catalog Number: LS-G117110-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human eIF4GII(EIF4G3) (NM_003760), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EIF4G3 | EIF-4-gamma II | EIF4G 3 | EIF-4-gamma 3 | EIF-4G 3 | EIF4GII
Origin Species
Human
NCBI GenBank
NM_003760
SwissProt
O43432

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
176.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSQPQTRSP FFQRPQIQPP RATIPNSSPS IRPGAQTPTA VYQANQHIMM VNHLPMPYPV
61PQGPQYCIPQ YRHSGPPYVG PPQQYPVQPP GPGPFYPGPG PGDFPNAYGT PFYPSQPVYQ
121SAPIIVPTQQ QPPPAKREKK TIRIRDPNQG GKDITEEIMS GGGSRNPTPP IGRPTSTPTP
181PQQLPSQVPE HSPVVYGTVE SAHLAASTPV TAASDQKQEE KPKPDPVLKS PSPVLRLVLS
241GEKKEQEGQT SETTAIVSIA ELPLPPSPTT VSSVARSTIA APTSSALSSQ PIFTTAIDDR
301CELSSPREDT IPIPSLTSCT ETSDPLPTNE NDDDICKKPC SVAPNDIPLV SSTNLINEIN
361GVSEKLSATE SIVEIVKQEV LPLTLELEIL ENPPEEMKLE CIPAPITPST VPSFPPTPPT
421PPASPPHTPV IVPAAATTVS SPSAAITVQR VLEEDESIRT CLSEDAKEIQ NKIEVEADGQ
481TEEILDSQNL NSRRSPVPAQ IAITVPKTWK KPKDRTRTTE EMLEAELELK AEEELSIDKV
541LESEQDKMSQ GFHPERDPSD LKKVKAVEEN GEEAEPVRNG AESVSEGEGI DANSGSTDSS
601GDGVTFPFKP ESWKPTDTEG KKQYDREFLL DFQFMPACIQ KPEGLPPISD VVLDKINQPK
661LPMRTLDPRI LPRGPDFTPA FADFGRQTPG GRGVPLLNVG SRRSQPGQRR EPRKIITVSV
721KEDVHLKKAE NAWKPSQKRD SQADDPENIK TQELFRKVRS ILNKLTPQMF NQLMKQVSGL
781TVDTEERLKG VIDLVFEKAI DEPSFSVAYA NMCRCLVTLK VPMADKPGNT VNFRKLLLNR
841CQKEFEKDKA DDDVFEKKQK ELEAASAPEE RTRLHDELEE AKDKARRRSI GNIKFIGELF
901KLKMLTEAIM HDCVVKLLKN HDEESLECLC RLLTTIGKDL DFEKAKPRMD QYFNQMEKIV
961KERKTSSRIR FMLQDVIDLR LCNWVSRRAD QGPKTIEQIH KEAKIEEQEE QRKVQQLMTK
1021EKRRPGVQRV DEGGWNTVQG AKNSRVLDPS KFLKITKPTI DEKIQLVPKA QLGSWGKGSS
1081GGAKASETDA LRSSASSLNR FSALQPPAPS GSTPSTPVEF DSRRTLTSRG SMGREKNDKP
1141LPSATARPNT FMRGGSSKDL LDNQSQEEQR REMLETVKQL TGGVDVERNS TEAERNKTRE
1201SAKPEISAMS AHDKAALSEE ELERKSKSII DEFLHINDFK EAMQCVEELN AQGLLHVFVR
1261VGVESTLERS QITRDHMGQL LYQLVQSEKL SKQDFFKGFS ETLELADDMA IDIPHIWLYL
1321AELVTPMLKE GGISMRELTI EFSKPLLPVG RAGVLLSEIL HLLCKQMSHK KVGALWREAD
1381LSWKDFLPEG EDVHNFLLEQ KLDFIESDSP CSSEALSKKE LSAEELYKRL EKLIIEDKAN
1441DEQIFDWVEA NLDEIQMSSP TFLRALMTAV CKAAIIADSS TFRVDTAVIK QRVPILLKYL
1501DSDTEKELQA LYALQASIVK LDQPANLLRM FFDCLYDEEV ISEDAFYKWE SSKDPAEQNG
1561KGVALKSVTA FFTWLREAEE ESEDN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1585 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.