Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EIF4G1 / EIF4G Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116292-100
Catalog Number: LS-G116292
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EIF4G1 (NM_004953), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EIF4-gamma | EIF-4-gamma 1 | EIF-4G1 | EIF4F | EIF4G | EIF4G1 | p220 | PARK18 | EIF-4G 1 | EIF4GI
- Origin Species
- Human
- NCBI GenBank
- NM_004953
- SwissProt
- Q04637
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 154.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGARTASTP TPPQTGGGLE PQANGETPQV AVIVRPDDRS QGAIIADRPG LPGPEHSPSE61SQPSSPSPTP SPSPVLEPGS EPNLAVLSIP GDTMTTIQMS VEESTPISRE TGEPYRLSPE121PTPLAEPILE VEVTLSKPVP ESEFSSSPLQ APTPLASHTV EIHEPNGMVP SEDLEPEVES181SPELAPPPAC PSESPVPIAP TAQPEELLNG APSPPAVDLS PVSEPEEQAK EVTASVAPPT241IPSATPATAP SATSPAQEEE MEEEEEEEEG EAGEAGEAES EKGGEELLPP ESTPIPANLS301QNLEAAAATQ VAVSVPKRRR KIKELNKKEA VGDLLDAFKE ANPAVPEVEN QPPAGSNPGP361ESEGSGVPPR PEEADETWDS KEDKIHNAEN IQPGEQKYEY KSDQWKPLNL EEKKRYDREF421LLGFQFIFAS MQKPEGLPHI SDVVLDKANK TPLRPLDPTR LQGINCGPDF TPSFANLGRT481TLSTRGPPRG GPGGELPRGP AGLGPRRSQQ GPRKEPRKII ATVLMTEDIK LNKAEKAWKP541SSKRTAADKD RGEEDADGSK TQDLFRRVRS ILNKLTPQMF QQLMKQVTQL AIDTEERLKG601VIDLIFEKAI SEPNFSVAYA NMCRCLMALK VPTTEKPTVT VNFRKLLLNR CQKEFEKDKD661DDEVFEKKQK EMDEAATAEE RGRLKEELEE ARDIARRRSL GNIKFIGELF KLKMLTEAIM721HDCVVKLLKN HDEESLECLC RLLTTIGKDL DFEKAKPRMD QYFNQMEKII KEKKTSSRIR781FMLQDVLDLR GSNWVPRRGD QGPKTIDQIH KEAEMEEHRE HIKVQQLMAK GSDKRRGGPP841GPPISRGLPL VDDGGWNTVP ISKGSRPIDT SRLTKITKPG SIDSNNQLFA PGGRLSWGKG901SSGGSGAKPS DAASEAARPA TSTLNRFSAL QQAVPTESTD NRRVVQRSSL SRERGEKAGD961RGDRLERSER GGDRGDRLDR ARTPATKRSF SKEVEERSRE RPSQPEGLRK AASLTEDRDR1021GRDAVKREAA LPPVSPLKAA LSEEELEKKS KAIIEEYLHL NDMKEAVQCV QELASPSLLF1081IFVRHGVEST LERSAIAREH MGQLLHQLLC AGHLSTAQYY QGLYEILELA EDMEIDIPHV1141WLYLAELVTP ILQEGGVPMG ELFREITKPL RPLGKAASLL LEILGLLCKS MGPKKVGTLW1201REAGLSWKEF LPEGQDIGAF VAEQKVEYTL GEESEAPGQR ALPSEELNRQ LEKLLKEGSS1261NQRVFDWIEA NLSEQQIVSN TLVRALMTAV CYSAIIFETP LRVDVAVLKA RAKLLQKYLC1321DEQKELQALY ALQALVVTLE QPPNLLRMFF DALYDEDVVK EDAFYSWESS KDPAEQQGKG1381VALKSVTAFF KWLREAEEES DHNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1403 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.