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EIF3D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EIF3D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EIF3D antibodies →
$504.00each
Catalog Number: LS-G100121-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EIF3D (NM_003753), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EIF3D | EIF3-zeta | EIF3 p66 | EIF3-p66 | EIF-3-zeta | EIF3S7
Origin Species
Human
NCBI GenBank
NM_003753
SwissProt
O15371

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKFMTPVIQ DNPSGWGPCA VPEQFRDMPY QPFSKGDRLG KVADWTGATY QDKRYTNKYS
61SQFGGGSQYA YFHEEDESSF QLVDTARTQK TAYQRNRMRF AQRNLRRDKD RRNMLQFNLQ
121ILPKSAKQKE RERIRLQKKF QKQFGVRQKW DQKSQKPRDS SVEVRSDWEV KEEMDFPQLM
181KMRYLEVSEP QDIECCGALE YYDKAFDRIT TRSEKPLRSI KRIFHTVTTT DDPVIRKLAK
241TQGNVFATDA ILATLMSCTR SVYSWDIVVQ RVGSKLFFDK RDNSDFDLLT VSETANEPPQ
301DEGNSFNSPR NLAMEATYIN HNFSQQCLRM GKERYNFPNP NPFVEDDMDK NEIASVAYRY
361RRWKLGDDID LIVRCEHDGV MTGANGEVSF INIKTLNEWD SRHCNGVDWR QKLDSQRGAV
421IATELKNNSY KLARWTCCAL LAGSEYLKLG YVSRYHVKDS SRHVILGTQQ FKPNEFASQI
481NLSVENAWGI LRCVIDICMK LEEGKYLILK DPNKQVIRVY SLPDGTFSSD EDEEEEEEEE
541EEEEEEET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

548 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.