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EIF2B4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EIF2B4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EIF2B4 antibodies →
$725.00each
Catalog Number: LS-G105499-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EIF2B4 (NM_172195), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP586J0119 | EIF2BD | EIF-2B | EIF2B | EIF2B4 | EIF2Bdelta
Origin Species
Human
NCBI GenBank
NM_015636
SwissProt
Q9UI10

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MPTQQPAAPS TRAPKPSRSL SGSLCALFSD ADSGSGMKAE LPPGPGAVGR EMTKEEKLQL
61RKEKKQQKKK RKEEKGAEPE TGSAVSAAQC QGPTRELPES GIQLGTPREK VPAGRSKAEL
121RAERRAKQEA ERALKQARKG EQGGPPPKAS PSTAGETPSG VKRLPEYPQV DDLLLRRLVK
181KPERQQVPTR KDYGSKVSLF SHLPQYSRQN SLTQFMSIPS SVIHPAMVRL GLQYSQGLVS
241GSNARCIALL RALQQVIQDY TTPPNEELSR DLVNKLKPYM SFLTQCRPLS ASMHNAIKFL
301NKEITSVGSS KREEEAKSEL RAAIDRYVQE KIVLAAQAIS RFAYQKISNG DVILVYGCSS
361LVSRILQEAW TEGRRFRVVV VDSRPWLEGR HTLRSLVHAG VPASYLLIPA ASYVLPEVSK
421VLLGAHALLA NGSVMSRVGT AQLALVARAH NVPVLVCCET YKFCERVQTD AFVSNELDDP
481DDLQCKRGEH VALANWQNHA SLRLLNLVYD VTPPELVDLV ITELGMIPCS SVPVVLRVKS
541SDQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

543 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.