Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EIF2A / EIF2 Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109053-100
Catalog Number: LS-G109053
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EIF2A (NM_032025), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDA02 | EIF2 alpha | EIF2A | MSTP004 | MSTP089 | EIF-2A | MST089
- Origin Species
- Human
- NCBI GenBank
- NM_032025
- SwissProt
- Q9BY44
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPSTPLLTV RGSEGLYMVN GPPHFTESTV FPRESGKNCK VCIFSKDGTL FAWGNGEKVN61IISVTNKGLL HSFDLLKAVC LEFSPKNTVL ATWQPYTTSK DGTAGIPNLQ LYDVKTGTCL121KSFIQKKMQN WCPSWSEDET LCARNVNNEV HFFENNNFNT IANKLHLQKI NDFVLSPGPQ181PYKVAVYVPG SKGAPSFVRL YQYPNFAGPH AALANKSFFK ADKVTMLWNK KATAVLVIAS241TDVDKTGASY YGEQTLHYIA TNGESAVVQL PKNGPIYDVV WNSSSTEFCA VYGFMPAKAT301IFNLKCDPVF DFGTGPRNAA YYSPHGHILV LAGFGNLRGQ MEVWDVKNYK LISKPVASDS361TYFAWCPDGE HILTATCAPR LRVNNGYKIW HYTGSILHKY DVPSNAELWQ VSWQPFLDGI421FPAKTITYQA VPSEVPNEEP KVATAYRPPA LRNKPITNSK LHEEEPPQNM KPQSGNDKPL481SKTALKNQRK HEAKKAAKQE ARSDKSPDLA PTPAPQSTPR NTVSQSISGD PEIDKKIKNL541KKKLKAIEQL KEQAATGKQL EKNQLEKIQK ETALLQELED LKLGITRTRPLEQKLISEEDLAANDILDYKDDDDKV
585 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.