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EGFLAM Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EGFLAM Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EGFLAM antibodies →
$725.00each
Catalog Number: LS-G106294-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EGFLAM (NM_152403), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
110.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLIRGVLLR LLLLASSLGP GAVSLRAAIR KPGKVGPPLD IKLGALNCTA FSIQWKMPRH
61PGSPILGYTV FYSEVGADKS LQEQLHSVPL SRDIPTTEEV IGDLKPGTEY RVSIAAYSQA
121GKGRLSSPRH VTTLSQDSCL PPAAPQQPHV IVVSDSEVAL SWKPGASEGS APIQYYSVEF
181IRPDFDKKWT SIHERIQMDS MVIKGLDPDT NYQFAVRAMN SHGPSPRSWP SDIIRTLCPE
241EAGSGRYGPR YITDMGAGED DEGFEDDLDL DISFEEVKPL PATKGGNKKF LVESKKMSIS
301NPKTISRLIP PTSASLPVTT VAPQPIPIQR KGKNGVAIMS RLFDMPCDET LCSADSFCVN
361DYTWGGSRCQ CTLGKGGESC SEDIVIQYPQ FFGHSYVTFE PLKNSYQAFQ ITLEFRAEAE
421DGLLLYCGEN EHGRGDFMSL AIIRRSLQFR FNCGTGVAII VSETKIKLGG WHMVMLYRDG
481LNGLLQLNNG TPVTGQSQGQ YSKITFRTPL YLGGAPSAYW LVRATGTNRG FQGCVQSLAV
541NGRRIDMRPW PLGKALSGAD VGECSSGICD EASCIHGGTC TAIKADSYIC LCPLGFKGRH
601CEDAFTLTIP QFRESLRSYA ATPWPLEPQH YLSFMEFEIT FRPDSGDGVL LYSYDTGSKD
661FLSINLAGGH VEFRFDCGSG TGVLRSEDPL TLGNWHELRV SRTAKNGILQ VDKQKIVEGM
721AEGGFTQIKC NTDIFIGGVP NYDDVKKNSG VLKPFSGSIQ KIILNDRTIH VKHDFTSGVN
781VENAAHPCVR APCAHGGSCR PRKEGYDCDC PLGFEGLHCQ KAIIEAIEIP QFIGRSYLTY
841DNPDILKRVS GSRSNVFMRF KTTAKDGLLL WRGDSPMRPN SDFISLGLRD GALVFSYNLG
901SGVASIMVNG SFNDGRWHRV KAVRDGQSGK ITVDDYGART GKSPGMMRQL NINGALYVGG
961MKEIALHTNR QYMRGLVGCI SHFTLSTDYH ISLVEDAVDG KNINTCGAK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1009 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.