Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EGFL8 / NG3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109443-100
Catalog Number: LS-G109443
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EGFL8 (NM_030652), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EGFL8 | EGF-like-domain | multiple 8 | EGF-like protein 8 | NG3 | VE-statin-2 | Vascular endothelial statin-2 | C6orf8
- Origin Species
- Human
- NCBI GenBank
- NM_030652
- SwissProt
- Q99944
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSRAELCTL LGGFSFLLLL IPGEGAKGGS LRESQGVCSK QTLVVPLHYN ESYSQPVYKP61YLTLCAGRRI CSTYRTMYRV MWREVRREVQ QTHAVCCQGW KKRHPGALTC EAICAKPCLN121GGVCVRPDQC ECAPGWGGKH CHVDVDECRT SITLCSHHCF NTAGSFTCGC PHDLVLGVDG181RTCMEGSPEP PTSASILSVA VREAEKDERA LKQEIHELRG RLERLEQWAG QAGAWVRAVL241PVPPEELQPE QVAELWGRGD RIESLSDQVL LLQERLGACS CEDNSLGLGV NHRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
293 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.