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EFR3A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EFR3A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113440-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EFR3A (NM_015137), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EFR3 homolog A (S. cerevisiae) | Protein EFR3-like | Protein EFR3 homolog A | EFR3A | KIAA0143
Origin Species
Human
NCBI GenBank
D63477
SwissProt
Q14156

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPTRVCCCCS ALRPRYKRLV DNIFPEDPKD GLVKTDMEKL TFYAVSAPEK LDRIGSYLAE
61RLSRDVVRHR SGYVLIAMEA LDQLLMACHS QSIKPFVESF LHMVAKLLES GEPKLQVLGT
121NSFVKFANIE EDTPSYHRRY DFFVSRFSAM CHSCHSDPEI RTEIRIAGIR GIQGVVRKTV
181NDELRATIWE PQHMDKIVPS LLFNMQKIEE VDSRIGPPSS PSATDKEENP AVLAENCFRE
241LLGRATFGNM NNAVRPVFAH LDHHKLWDPN EFAVHCFKII MYSIQAQYSH HVIQEILGHL
301DARKKDAPRV RAGIIQVLLE AVAIAAKGSI GPTVLEVFNT LLKHLRLSVE FEANDLQGGS
361VGSVNLNTSS KDNDEKIVQN AIIQTIGFFG SNLPDYQRSE IMMFIMGKVP VFGTSTHTLD
421ISQLGDLGTR RIQIMLLRSL LMVTSGYKAK TIVTALPGSF LDPLLSPSLM EDYELRQLVL
481EVMHNLMDRH DNRAKLRGIR IIPDVADLKI KREKICRQDT SFMKKNGQQL YRHIYLGCKE
541EDNVQKNYEL LYTSLALITI ELANEEVVID LIRLAIALQD SAIINEDNLP MFHRCGIMAL
601VAAYLNFVSQ MIAVPAFCQH VSKVIEIRTM EAPYFLPEHI FRDKCMLPKS LEKHEKDLYF
661LTNKIAESLG GSGYSVERLS VPYVPQVTDE DRLSRRKSIV DTVSIQVDIL SNNVPSDDVV
721SNTEEITFEA LKKAIDTSGM EEQEKEKRRL VIEKFQKAPF EEIAAQCESK ANLLHDRLAQ
781ILELTIRPPP SPSGTLTITS GHAQYQSVPV YEMKFPDLCV Y
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

821 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.