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EFHC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EFHC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G130093-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EFHC1 (NM_001172420), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ304B14.2 | EJA1 | EJM1 | Myoclonin-1 | EFHC1 | JAE
Origin Species
Human
NCBI GenBank
NM_018100
SwissProt
Q5JVL4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLKTAFHRSQ TLSYRNGYAI VRRPTVGIGG DRLQFNQLSQ AELDELASKA PVLTYGQPKQ
61APPADFIPAH VAFDKKVLKF DAYFQEDVPM STEEQYRIRQ VNIYYYLEDD SMSVIEPVVE
121NSGILQGKLI KRQRLAKNDR GDHYHWKDLN RGINITIYGK TFRVVDCDQF TQVFLESQGI
181ELNPPEKMAL DPYTELRKQP LRKYVTPSDF DQLKQFLTFD KQVLRFYAIW DDTDSMYGEC
241RTYIIHYYLM DDTVEIREVH ERNDGRDPFP LLMNRQRVPK VLVENAKNFP QCVLEISDQE
301VLEWYTAKDF IVGKSLTILG RTFFIYDCDP FTRRYYKEKF GITDLPRIDV SKREPPPVKQ
361ELPPYNGFGL VEDSAQNCFA LIPKAPKKDV IKMLVNDNKV LRYLAVLESP IPEDKDRRFV
421FSYFLATDMI SIFEPPVRNS GIIGGKYLGR TKVVKPYSTV DNPVYYGPSD FFIGAVIEVF
481GHRFIILDTD EYVLKYMESN AAQYSPEALA SIQNHVRKRE APAPEAESKQ TEKDPGVQEL
541EALIDTIQKQ LKDHSCKDNI REAFQIYDKE ASGYVDRDMF FKICESLNVP VDDSLVKELI
601RMCSHGEGKI NYYNFVRAFS N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

621 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.