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EFHB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EFHB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EFHB antibodies →
$504.00each
Catalog Number: LS-G106880-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EFHB (NM_144715), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EFHB
Origin Species
Human
NCBI GenBank
NM_144715
SwissProt
Q8N7U6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPRQEMEKE STCVLMKPNT EIKLPVEVDI GLTQAEGPDE TKNTEPQMGL VIEPPQCQFA
61QQHEQRKEAG NIESGVEPPD RIRPIYSGKF FDRTPCWPSA GKVIPVGYRV ATCLTEKLPR
121LITPPEAKKY FNFRYPPAGV ERVFYGRAND PQIAPYLTHG IRSKISILAN TLINPQPITT
181FQQKIKDKKE SIYLSNRRAP LGKSHDQAPG LPKGMDTINT TFGTAVIKEY SAKDVVNPPK
241SYEEVFKEGN EGHDLYVVSH NDYYAGEAKN RKYNPSSFHR CSVYGVPTPH FNDGRAMAKS
301LYWLHELQMK RGAKFVSKRA DDFKEKFQHK LGRVLDPIAE TMNVPPDCTF GACLRPEEYG
361VGDLIHNRLP DEYLRGKDRQ RALIAAVRHH LKKVNYQKFD TLLAAFRHYD KKGDGMIDKD
421ELQEACDQAN LSLDDKLLDQ LFDYCDVDND GFINYLEFAN FLNWKDKMLL KEYEERVIIK
481GRKPDCVNPT EANVEEPEQT LLIKPEDIVL KEAGSTEKTL RTLLRPSDKV SNYYKTTSSE
541INAIVGAIPS TCYPICGVPT IRSDIPAPRI RRISDRTNYG EEGSAYSLLY PTIFARKGVF
601ERDFFKTRSK EEIAEILCNI GVKLSDEEFE NVWNLASKKH HRGEVCVENI RNVLDELRHA
661DRIKCKTLM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

669 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.