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EFCAB6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EFCAB6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EFCAB6 antibodies →
$504.00each
Catalog Number: LS-G103529-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EFCAB6 (NM_198856), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ-1-binding protein | DJBP | EFCAB6 | HSCBCIP1 | KIAA1672 | DJ185D5.1 | FLJ23588
Origin Species
Human
NCBI GenBank
NM_022785
SwissProt
Q5THR3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
155.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNCCRTLREL EIQVGEKVFK NIKTVMKAFE LIDVNKTGLV RPQELRRVLE TFCMKLRDEE
61YEKFSKHYNI HKDTAVDYNV FLKNLSINND LNLRYCMGNQ EVSLENQQAK NSKKERLLGS
121ASSEDIWRNY SLDEIERNFC LQLSKSYEKV EKALSAGDPC KGGYVSFNYL KIVLDTFVYQ
181IPRRIFIQLM KRFGLKATTK INWKQFLTSF HEPQGLQVSS KGPLTKRNSI NSRNESHKEN
241IITKLFRHTE DHSASLKKAL LIINTKPDGP ITREEFRYIL NCMAVKLSDS EFKELMQMLD
301PGDTGVVNTS MFIDLIEENC RMRKTSPCTD AKTPFLLAWD SVEEIVHDTI TRNLQAFYNM
361LRSYDLGDTG RIGRNNFKKI MHVFCPFLTN AHFIKLCSKI QDIGSGRILY KKLLACIGID
421GPPTVSPVLV PKDQLLSEHL QKDEQQQPDL SERTKLTEDK TTLTKKMTTE EVIEKFKKCI
481QQQDPAFKKR FLDFSKEPNG KINVHDFKKV LEDTGMPMDD DQYALLTTKI GFEKEGMSYL
541DFAAGFEDPP MRGPETTPPQ PPTPSKSYVN SHFITAEECL KLFPRRLKES FRDPYSAFFK
601TDADRDGIIN MHDLHRLLLH LLLNLKDDEF ERFLGLLGLR LSVTLNFREF QNLCEKRPWR
661TDEAPQRLIR PKQKVADSEL ACEQAHQYLV TKAKNRWSDL SKNFLETDNE GNGILRRRDI
721KNALYGFDIP LTPREFEKLW ARYDTEGKGH ITYQEFLQKL GINYSPAVHR PCAEDYFNFM
781GHFTKPQQLQ EEMKELQQST EKAVAARDKL MDRHQDISKA FTKTDQSKTN YISICKMQEV
841LEECGCSLTE GELTHLLNSW GVSRHDNAIN YLDFLRAVEN SKSTGAQPKE KEESMPINFA
901TLNPQEVVRK IQEVVESSQL ALSTAFSALG KEDTGFVKAT EFGQVLKDFC YKLTDNQYHY
961FLRKLRIHLT PYINWKYFLQ NFSCFLEETA DEWAEKMPKG PPPTSPKATA DRDILARLHK
1021AVTSHYHAIT QEFENFDTMK TNTISREEFR AICNRRVQIL TDEQFGRLWN EMPVNAKGRL
1081KYPDFLSRFS SETAATPMAT GDSAVAQRGS SVPDVSEGTR SALSLPTQEL RPGSKSQSHP
1141CTPASTTVIP GTPPLQNCDP IESRLRKRIQ GCWRQLLKEC KEKDVARQGD INASDFLALV
1201EKFNLDISKE ECQQLIIKYD LKSNGKFAYC DFIQSCVLLL KAKESSLMHR MKIQNAHKMK
1261EAGAETPSFY SALLRIQPKI VHCWRPMRRT FKSYDEAGTG LLSVADFRTV LRQYSINLSE
1321EEFFHILEYY DKTLSSKISY NDFLRAFLQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1349 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.