LSBio
EFCAB5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EFCAB5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EFCAB5 antibodies →
$725.00each
Catalog Number: LS-G103636-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EFCAB5 (NM_198529), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
173.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNESASQEEL RPAQENRKED KERKWNLTEV KELHETLQSV PDVPVKEDTN SVVEKAMDEI
61KSQELNLEGQ RKISPGSIKD SKTEASGNIA IRKSAKVIFA LDETELKSKP EHTWKKNLFE
121RMEARAQAMQ QKIIDKENLK KELEKKAEKK LPRDNLAKEW FNTDSMTLNN TAYLLDKLLP
181TLVPGVENML TQVEKKKVLT EADTPSKFDP INYLGEYLIR NNPNYIKDPG MSGYQRLMKE
241VTEDLKIYVP DTICNRVSKM KENVKQNRKQ RESIDKIKVK VANTRKQALQ EQFDEWILDP
301KGMIPKSVIQ NVLQEFFQNP DFKLGSHCKQ LDITDSTEPR LNKMEFTEYI SSHIKDLKSE
361MFEELLKHLC HSADEFREVI KADMRRQMFA ELFLHCDHGK VGFLDRQRTL ALLELFYDHS
421SQMLRSLLRN PRQWPFIEFE EINLTELWGD MDNQKHIYEG FDKVLLEMNT LLSANHASKT
481QSKLLESPDQ PKLNEQRTST PSPNPPEQQR GVTAEQGPQR ISIEEQQQGK KPTAEQELYI
541ESVIEPGTHT ESTLEQGSSR RLLTEQETHR ESTTEQGQHK GSIEGQGPRR VSVSEQGSSR
601ESVAEQGSRR ESIAEQDRHK GSVAEQGSRR MSAAEQGSLR ESVIEEPYQK SEQGPYGEII
661SEEQEDIGST SQSRKDSILK STKYGEPITS EYIEVPLQEK RSWEQTYEEE IFLSSELQEE
721VPTLSRKDHF PETTKKEVQK DKPCEPKSQK IEGKSWSGEF FTCNWKMKYV TFEDEEQANL
781IYGNSRFTDL HSIIRNIQSC KEVKGRTAFN GVSFNLLQFV QLLETFVGED APLSVSETLT
841SFFKEGYVET EQEKMNALEQ FSQNAFQVRQ RLLLEAIFQK WDSDGSGFLD LKEVDELLYT
901YKEGMEKESM KKAKLHIQFP KPHPGHEVRL SSKQFQNYIE LVVSELRGNE DQVLESVVEF
961LMNALERSHI ESLRNSARRK WLHQIQCAAE TSGVSLEPVY SETFKALMQD AEAHGNKKIS
1021AHISLLEENL LLPEKGNVLL RNVACTLDDA QFVLNRVLYR DMKGISFTVV DEGKPIHVPQ
1081VQYHGNIFFW NQSRNKHDYN GSFLALPLQD AYMRIFGVLA VDTLRDPHEI NIFLPHEIRF
1141YQGVANVFST AYHYVHSREH ILHIVITGIG WLYDVTSSIT SITTYFVEPS PAQDSDYVLR
1201NMMVTGQLGL TEIHKNPPTI HRKSCIFRDF LFKCTDSSEV VLASACGETH IVVPLRERTG
1261EALGVLDFNI GQNRMLLCQE YKDLQKMMKV VQVACYEILG EFSGEIKKKY ILEIENVREV
1321QRAGILFFRI MLLELQESIQ LLNSMEFVSL LLYDHTLVTE PNSPQDSKSM ELEANVKLVR
1381DILKAVILFF HPELEFSSDF GSWDKCKFYV NKYLVNNICA FDPTAKHVEV NVQLIDEYIR
1441DHSRTEVWKF GNVVIEHLYH WIHICSALMK ITKQLNSGIT PPLPSKTDNY MYAKMPGEGL
1501QEK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1503 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.