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EFCAB14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EFCAB14 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EFCAB14 antibodies →
$504.00each
Catalog Number: LS-G113706-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0494(EFCAB14) (NM_014774), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Efcab14 | KIAA0494 | RP11-8J9.3
Origin Species
Human
NCBI GenBank
NM_014774
SwissProt
O75071

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKKRKELNAL IGLAGDSRRK KPKKGPSSHR LLRTEPPDSD SESSSEEEEE FGVVGNRSRF
61AKGDYLRCCK ICYPLCGFVI LAACVVACVG LVWMQVALKE DLDALKEKFR TMESNQKSSF
121QEIPKLNEEL LSKQKQLEKI ESGEMGLNKV WINITEMNKQ ISLLTSAVNH LKANVKSAAD
181LISLPTTVEG LQKSVASIGN TLNSVHLAVE ALQKTVDEHK KTMELLQSDM NQHFLKETPG
241SNQIIPSPSA TSELDNKTHS ENLKQDILYL HNSLEEVNSA LVGYQRQNDL KLEGMNETVS
301NLTQRVNLIE SDVVAMSKVE KKANLSFSMM GDRSATLKRQ SLDQVTNRTD TVKIQSIKKE
361DSSNSQVSKL REKLQLISAL TNKPESNRPP ETADEEQVES FTSKPSALPK FSQFLGDPVE
421KAAQLRPISL PGVSSTEDLQ DLFRKTGQDV DGKLTYQEIW TSLGSAMPEP ESLRAFDSDG
481DGRYSFLELR VALGITRTRP LEQKLISEED LAANDILDYK DDDDKV

526 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.