Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EFCAB12 / C3orf25 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102867-100
Catalog Number: LS-G102867
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C3orf25(EFCAB12) (NM_207307), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_207307
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDDDYEAYHS LFLSLLGLCP SKTPINENAP VFDPEPVIAH CFKQFQQKDF RLPQTRRRII61MVPRKEDQTP LNPASQPQAP PKPIPSFKVL EARDIQEQPE DRKTWLSQRS KLRQELESFG121DVKRWLENKP SITPSEAKVL HMIHEEQSAQ PNASQATTRT TRKKAPRLSR LSRQMVPQLQ181LPEPPALSVM YSYLHSRKIK ILEIFHKVGQ GENQRITREE FIAAVKAVGV PLKNQEVEDI241VIYLSSLGKH NTITMDILAN TYKQWSMAQQ RSSLATAREH YILAKHRDSL KGPLKKQEVD301SAPQLPKVDL LTVPAVDTQM ETRPMTLEEM EEVGKRYRER QRQHKLTIPS IQYTEQCHLV361RCGNRHFDEH CLPSTIHGDM RELIDSARRH NFLVYLQCWK LCKSYGLPLT EDILMKALLY421PGDKIIFQMD KVCPIRQPGG YYSDWKVFSP NLALLRSQGP GKSKRTDKKT PKKSKKMRFK481EFEEFTRKLK VKRSSGLQQT HPNSFWPGHL LDKLQLYLPT VATDRSLALF SCVQHQPHVY541PATYHPDHWW PLRNKNYMTH AHYDAAKVYY INTRTRPLEQKLISEEDLAANDILDYKDDDDKV
572 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.