Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EEYORE / TIGD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106621-100
Catalog Number: LS-G106621
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TIGD1 (NM_145702), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EEYORE | TIGD1
- Origin Species
- Human
- SwissProt
- Q96MW7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASKCSSERK SRTSLTLNQK LEMIKLSEEG MSKAEIGRRL GLLRQTVSQV VNAKEKFLKE61VKSATPMNTR MIRKRNSLIA DMEKVLVVWI EDQTSRNIPL SQSLIQNKAL TLFNSMKAER121GVEAAEEKFE ASRGWFMRFK ERSHFHNIKA QGEAASADVE AAASYPEALA KIIDEGGYTK181QQIFNVDETA FYWKKMPSRT FIAREEKSVP GFKASKDRLT LLLGANAAGD FKLKPMLIYH241SENPRALKSY TKSTLPVLYK WNSKARMTAH LFTAWFTEYF KPTVETYCSE KKIPFKILLL301IGNAPSHPRA LMEIYEEINV IFMPANTTSI LQPMDQGVIS TFKSYYLRNT FHKALAAMDS361DVSDGSGQSK LKTFWKGFTI LDAIKNIRDS WEEVKLSTLT GVWKKLIPTL IDDYEGFKTS421VEEVSADVVE IAKELELEVE PEDVTELLQS HDKTLTDEEL FLMDAQRKWF LEMESTPGED481AVNIVEMTTK DLEYYINLVD KAAAGFERID SNFERSSTVG KMLSNSIACY REIFHERKSQ541LMRKASPMSY FRKLPQPPQP SAATTLTSQQ PSTSRQDPPP AKRVRLTEGS DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
591 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.