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EEPD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EEPD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EEPD1 antibodies →
$504.00each
Catalog Number: LS-G109449-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EEPD1 (NM_030636), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EEPD1 | HSPC107 | KIAA1706
Origin Species
Human
SwissProt
Q3TGW2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSTLGCHRS IPRDPSDLSH SRKFSAACNF SNILVNQERL NINTATEEEL MTLPGVTRAV
61ARSIVEYREY IGGFKKVEDL ALVSGVGATK LEQVKFEICV SSKGSSAQHS PSSLRRDLLA
121EQQPHHLATA VPLTPRVNIN TATPAQLMSV RGLSEKMALS IVDFRREHGP FRSVEDLVRM
181DGINAAFLDR IRHQVFAERS RPPSTHTNGG LTFTAKPHPS PTSLSLQSED LDLPPGGPTQ
241IISTRPSVEA FGGTRDGRPV LRLATWNLQG CSVEKANNPG VREVVCMTLL ENSIKLLAVQ
301ELLDREALEK FCTELNQPTL PNIRKWKGPR GCWKAVVAEK PSNQLQKGAG YAGFLWDAAA
361GMELRDAGSQ ESSPSNGHGK LAGPSPYLGR FKVGSHDLTL VNLHLAALTL LGSENPSKNH
421SDGHRLASFA QTLQETLKGE KDVIILGDFG QGPDSNDYDI LRKEKFHHLI PAHTFTNIST
481KNPQGSKSLD NIWISKSLKK VFTGHWAVVR EGLTNPWIPD NWSWGGVASE HCPVLAEFYT
541EKDWSKKDAP RNGSGVALER SEANIKHER
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

569 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.