Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EEFSEC / EFSEC Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110556-100
Catalog Number: LS-G110556
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EEFSEC (NM_021937), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EFSEC | Elongation factor sec | SELB | EEFSEC
- Origin Species
- Human
- SwissProt
- P57772
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGRRVNVNV GVLGHIDSGK TALARALSTT ASTAAFDKQP QSRERGITLD LGFSCFSVPL61PARLRSSLPE FQAAPEAEPE PGEPLLQVTL VDCPGHASLI RTIIGGAQII DLMMLVIDVT121KGMQTQSAEC LVIGQIACQK LVVVLNKIDL LPEGKRQAAI DKMTKKMQKT LENTKFRGAP181IIPVAAKPGG PEAPETEAPQ GIPELIELLT SQISIPTRDP SGPFLMSVDH CFSIKGQGTV241MTGTILSGSI SLGDSVEIPA LKVVKKVKSM QMFHMPITSA MQGDRLGICV TQFDPKLLER301GLVCAPESLH TVHAALISVE KIPYFRGPLQ TKAKFHITVG HETVMGRLMF FSPAPDNFDQ361EPILDSFNFS QEYLFQEQYL SKDLTPAVTD NDEADKKAGQ ATEGHCPRQQ WALVEFEKPV421TCPRLCLVIG SRLDADIHTN TCRLAFHGIL LHGLEDRNYA DSFLPRLKVY KLKHKHGLVE481RAMDDYSVIG RSLFKKETNI QLFVGLKVHL STGELGIIDS AFGQSGKFKI HIPGGLSPES541KKILTPALKK RARAGRGEAT RQEESAERSE PSQHVVLSLT FKRYVFDTHK RMVQSPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
596 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.