Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EDRF1 / C10orf137 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113124-100
Catalog Number: LS-G113124
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C10orf137(EDRF1) (NM_015608), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EDRF1 | C10orf137
- Origin Species
- Human
- NCBI GenBank
- W87631
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 134.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGDAKEAGAE GPPAGAAARG GLSLLSQGES EESSAQGSAL FLGGNEVKSR AVVKYSSAPP61RTAFARLEEK TDLKLPPANW LRESAKLGPA GTTILGNSKK SKPFSSFGMA YDFIDSVGND121VDVVSDSENI KKLLKIPYSK SHVSMAVHRI GRTLLLDELD IQELFMRSSQ TGDWTWLKEF181YQRLIDQKWQ RKKKSKEHWY QKAILSKFLY YSINGDGAAQ PVSSTAEQQE SSSSDQTNDS241EGASWPAPFE MPSSVSEDPS ASSQGLKNDF VRNILWTFED IHMLVGSNMP IFGGGRYPAV301SLRLRDNNKP INVLTGIDYW LDNLICNVPE LVMCFHVNGI VQKYEMIKTE EIPNLENSNF361STKVIKDIAQ NILSFLKSNC TKEGHTYWLF KASGSDIVKL YDLTTLCEET EDKYQNPFTM421PVAILLYKVA CNMMMKKNQN KKHYGTIRTL LLNCLKLLDK SRHPQIIASA NYMLSELFQL481DEPKKEENSE SPLNENSDES YSEEEEEMPD SDENGSYSTS SDPSDDSKAV AIIKSVGELS541VPEKYKSIHQ IRPSCAFPVC HDTEERCRLV LSYVLEGLKS VDSSIKKESD LPAADPSTPI601PLKYEDESSR GGPEGLEKQM ALFLDKMGSL QKGNYSSQSG MIPGSWQHKM KLQLILKSSK661AYYVLSDAAM SLQKYGRALR YIKLALQSHD TYCCLCTNML SEVLLFLSQY LTLCGDIQLM721LAQNANNRAA HLEEFHYQTK EDQEILHSLH RESSCQGFAW ATDLSTDLES QLSVSCKCYE781AANEILQFSD LKSQNPEHYV QVLKRMGNIR NEIGVFYMNQ AAALQSERLV SKSVSAAEQQ841LWKKSFSCFE KGIHNFESIE DATNAALLLC NTGRLMRICA QAHCGAGDEL KREFSPEEGL901YYNKAIDYYL KALRSLGTRD IHPAVWDSVN WELSTTYFTM ATLQQDYAPL SRKAQEQIEK961EVSEAMMKSL KYCDVDSVSA RQPLCQYRAA TIHHRLASMY HSCLRNQVGD EHLRKQHRVL1021ADLHYSKAAK LFQLLKDAPC ELLRVQLERV AFAEFQMTSQ NSNVGKLKTL SGALDIMVRT1081EHAFQLIQKE LIEEFGQPKS GDAAAAADAS PSLNREEVMK LLSIFESRLS FLLLQSIKLL1141SSTKKKTSNN IEDDTILKTN KHIYSQLLRA TANKTATLLE RINVIVHLLG QLAAGSAASS1201NAVQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1204 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.