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EDEM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EDEM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EDEM2 antibodies →
$504.00each
Catalog Number: LS-G126482-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C20orf31(EDEM2) (NM_001145025), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA4204.1 | C20orf49 | EDEM2 | C20orf31
Origin Species
Human
NCBI GenBank
NM_018217
SwissProt
Q9BV94

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPFRLLIPLG LLCALLPQHH GAPGPDGSAP DPAHYSFSLT LIDALDTLLI LGNVSEFQRV
61VEVLQDSVDF DIDVNASVFE TNIRVVGGLL SAHLLSKKAG VEVEAGWPCS GPLLRMAEEA
121ARKLLPAFQT PTGMPYGTVN LLHGVNPGET PVTCTAGIGT FIVEFATLSS LTGDPVFEDV
181ARVALMRLWE SRSDIGLVGN HIDVLTGKWV AQDAGIGAGV DSYFEYLVKG AILLQDKKLM
241AMFLEYNKAI RNYTRFDDWY LWVQMYKGTV SMPVFQSLEA YWPGLQSLIG DIDNAMRTFL
301NYYTVWKQFG GLPEFYNIPQ GYTVEKREGY PLRPELIESA MYLYRATGDP TLLELGRDAV
361ESIEKISKVE CGFATIKDLR DHKLDNRMES FFLAETVKYL YLLFDPTNFI HNNGSTFDAV
421ITPYGECILG AGGYIFNTEA HPIDPAALHC CQRLKEEQWE VEDLMREFYS LKRSRSKFQK
481NTVSSGPWEP PARPGTLFSP ENHDQARERK PAKQKVPLLS CPSQPFTSKL ALLGQVFLDS
541S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

541 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.