Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EDAG / HEMGN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103864-100
Catalog Number: LS-G103864
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Hemogen(HEMGN) (NM_197978), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EDAG-1 | HEMGN | Hemogen | Hemopoietic gene protein | EDAG | NDR
- Origin Species
- Human
- NCBI GenBank
- NM_018437
- SwissProt
- Q9BXL5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDLGKDQSHL KHHQTPDPHQ EENHSPEVIG TWSLRNRELL RKRKAEVHEK ETSQWLFGEQ61KKRKQQRTGK GNRRGRKRQQ NTELKVEPQP QIEKEMEKAL APIEKKTEPP GSITKVFPSV121ASPQKVVPEE HFSEICQESN IYQENFSEYQ EIAVQNHSSE TCQHVSEPED LSPKMYQEIS181VLQDNSSKIC QDMKEPEDNS PNTCQVISVI QDHPFKMYQD MAKREDLAPK MCQEAAVPKI241LPCPTSEDTA DLAGCSLQAY PKPDVPKGYI LDTDQNPAEP EEYNETDQGI AETEGLFPKI301QEIAEPKDLS TKTHQESAEP KYLPHKTCNE IIVPKAPSHK TIQETPHSED YSIEINQETP361GSEKYSPETY QEIPGLEEYS PEIYQETSQL EEYSPEIYQE TPGPEDLSTE TYKNKDVPKE421CFPEPHQETG GPQGQDPKAH QEDAKDAYTF PQEMKEKPKE EPGIPAILNE SHPENDVYSY481VLFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
483 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.