LSBio
ECE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ECE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ECE2 antibodies →
$725.00each
Catalog Number: LS-G113776-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ECE2 (NM_014693), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ECE-2 | Endothelin-converting enzyme 2 | ECE2 | KIAA0604 | Endothelin converting enzyme 2
Origin Species
Human
NCBI GenBank
NM_014693
SwissProt
O60344

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASPGAGRAP PELPERNCGY REVEYWDQRY QGAADSAPYD WFGDFSSFRA LLEPELRPED
61RILVLGCGNS ALSYELFLGG FPNVTSVDYS SVVVAAMQAR HAHVPQLRWE TMDVRKLDFP
121SASFDVVLEK GTLDALLAGE RDPWTVSSEG VHTVDQVLSE VGFQKGTRQL LGSRTQLELV
181LAGASLLLAA LLLGCLVALG VQYHRDPSHS TCLTEACIRV AGKILESLDR GVSPCEDFYQ
241FSCGGWIRRN PLPDGRSRWN TFNSLWDQNQ AILKHLLENT TFNSSSEAEQ KTQRFYLSCL
301QVERIEELGA QPLRDLIEKI GGWNITGPWD QDNFMEVLKA VAGTYRATPF FTVYISADSK
361SSNSNVIQVD QSGLFLPSRD YYLNRTANEK VLTAYLDYME ELGMLLGGRP TSTREQMQQV
421LELEIQLANI TVPQDQRRDE EKIYHKMSIS ELQALAPSMD WLEFLSFLLS PLELSDSEPV
481VVYGMDYLQQ VSELINRTEP SILNNYLIWN LVQKTTSSLD RRFESAQEKL LETLYGTKKS
541CVPRWQTCIS NTDDALGFAL GSLFVKATFD RQSKEIAEGM ISEIRTAFEE ALGQLVWMDE
601KTRQAAKEKA DAIYDMIGFP DFILEPKELD DVYDGYEISE DSFFQNMLNL YNFSAKVMAD
661QLRKPPSRDQ WSMTPQTVNA YYLPTKNEIV FPAGILQAPF YARNHPKALN FGGIGVVMGH
721ELTHAFDDQG REYDKEGNLR PWWQNESLAA FRNHTACMEE QYNQYQVNGE RLNGRQTLGE
781NIADNGGLKA AYNAYKAWLR KHGEEQQLPA VGLTNHQLFF VGFAQVWCSV RTPESSHEGL
841VTDPHSPARF RVLGTLSNSR DFLRHFGCPV GSPMNPGQLC EVW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

883 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.