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ECD Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ECD Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G125540-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SGT1(ECD) (NM_001135752), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HSGT1 | GCR2 | Protein ecdysoneless homolog | Protein SGT1 | SGT1 | Suppressor of GCR2 | ECD
Origin Species
Human
NCBI GenBank
NM_007265
SwissProt
O95905

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEETMKLATM EDTVEYCLFL IPDESRDSDK HKEILQKYIE RIITRFAPML VPYIWQNQPF
61NLKYKPGKGG VPAHMFGVTK FGDNIEDEWF IVYVIKQITK EFPELVARIE DNDGEFLLIE
121AADFLPKWLD PENSTNRVFF CHGELCIIPA PRKSGAESWL PTTPPTIPQA LNIITAHSEK
181ILASESIRAA VNRRIRGYPE KIQASLHRAH CFLPAGIVAV LKQRPRLVAA AVQAFYLRDP
241IDLRACRVFK TFLPETRIMT SVTFTKCLYA QLVQQRFVPD RRSGYRLPPP SDPQYRAHEL
301GMKLAHGFEI LCSKCSPHFS DCKKSLVTAS PLWASFLESL KKNDYFKGLI EGSAQYRERL
361EMAENYFQLS VDWPERLEVQ WRDPGLLQAP PPGFTPFICL SLLSTWDNSS LAMSPGEEIL
421TLLQTIPFDI EDLKKEAANL PPEDDDQWLD LSPDQLDQLL QEAVGKKESE SVSKEEKEQN
481YDLTEVSESM KAFISKVSTH KGAELPREPS EAPITFDADS FLNYFDKILG PRPNESDSDD
541LDDEDFECLD SDDDLDFETH EPGEEASLKG TLDNLKSYMA QMDQELAHTC ISKSFTTRNQ
601VEPVSQTTDN NSDEEDSGTG ESVMAPVDVD LNLVSNILES YSSQAGLAGP ASNLLQSMGV
661QLPDNTDHRP TSKPTKN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

677 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.