Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EBF3 / COE3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122219-100
Catalog Number: LS-G122219
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EBF3 (NM_001005463), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COE3 | Early B-cell factor 3 | EBF3 | O/E-2 | Olf-1/EBF-like 2 | EBF-3 | OE-2 | Transcription factor COE3
- Origin Species
- Human
- NCBI GenBank
- NM_001005463
- SwissProt
- Q9H4W6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFGIQENIPR GGTTMKEEPL GSGMNPVRSW MHTAGVVDAN TAAQSGVGLA RAHFEKQPPS61NLRKSNFFHF VLALYDRQGQ PVEIERTAFV DFVEKEKEPN NEKTNNGIHY KLQLLYSNGV121RTEQDLYVRL IDSMTKQAIV YEGQDKNPEM CRVLLTHEIM CSRCCDKKSC GNRNETPSDP181VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH241GRRARRLDPS EATPCIKAIS PSEGWTTGGA TVIIIGDNFF DGLQVVFGTM LVWSELITPH301AIRVQTPPRH IPGVVEVTLS YKSKQFCKGA PGRFVYTALN EPTIDYGFQR LQKVIPRHPG361DPERLPKEVL LKRAADLVEA LYGMPHNNQE IILKRAADIA EALYSVPRNH NQIPTLGNNP421AHTGMMGVNS FSSQLAVNVS ETSQANDQVG YSRNTSSVSP RGYVPSSTPQ QSNYNTVSTS481MNGYGSGAMA SLGVPGSPGF LNGSSANSPY GMKQKSAFAP VVRPQASPPP SCTSANGNGL541QAMSGLVVPP MTRTRPLEQKLISEEDLAANDILDYKDDDDKV
551 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.